BMRB Entry 5366

Name: SOLUTION STRUCTURE OF THE VAM7P PX DOMAIN
Published: 2003-02-25

Click here to enlarge.

PDB ID: 1kmd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5366
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

SOLUTION STRUCTURE OF THE VAM7P PX DOMAIN

Deposition date:

2002-05-08

Original release date:

2003-02-25

Authors:

Lu, J.; Garcia, .; Dulubova, I.; Sudhof, T.; Rizo, J.

Citation:

Citation: Lu, J.; Garcia, J.; Dulubova, I.; Sudhof, T.; Rizo, J.. "Solution Structure of the Vam7P PX Domain" Biochemistry 41, 5956-5962 (2002).
PubMed: 11993989

Assembly members:

Assembly members:
vam7p, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: not available Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
vam7p: KMSEKLRKMSEKLRIKVDDV KINPKYVLYGVSTPNKRLYK RYSEFWKLKTRLERDVGSTI PYDFPEKPGVLDRRWQRRYD DPEMIDERRIGLERFLNELY NDRFDSRWRDTKIAQDFLQL SKPN

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	575
15N chemical shifts	127
1H chemical shifts	897

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Vacuolar morphogenesis protein	1

Entities:

Entity 1, Vacuolar morphogenesis protein 124 residues - Formula weight is not available

1

LYS

MET

SER

GLU

LYS

LEU

ARG

LYS

MET

SER

2

GLU

LYS

LEU

ARG

ILE

LYS

VAL

ASP

VAL

3

LYS

ILE

ASN

PRO

LYS

TYR

VAL

LEU

TYR

GLY

4

VAL

SER

THR

PRO

ASN

LYS

ARG

LEU

TYR

LYS

5

ARG

TYR

SER

GLU

PHE

TRP

LYS

LEU

LYS

THR

6

ARG

LEU

GLU

ARG

ASP

VAL

GLY

SER

THR

ILE

7

PRO

TYR

ASP

PHE

PRO

GLU

LYS

PRO

GLY

VAL

8

LEU

ASP

ARG

TRP

GLN

ARG

TYR

ASP

9

ASP

PRO

GLU

MET

ILE

ASP

GLU

ARG

ILE

10

GLY

LEU

GLU

ARG

PHE

LEU

ASN

GLU

LEU

TYR

11

ASN

ASP

ARG

PHE

ASP

SER

ARG

TRP

ARG

ASP

12

THR

LYS

ILE

ALA

GLN

ASP

PHE

LEU

GLN

LEU

13

SER

LYS

PRO

ASN

Samples:

sample_1: vam7p, [U-15N], 0.5 mM; Mes buffer 20 mM; H2O 90%; D2O 10%

sample_2: vam7p, [U-15N; U-13C], 0.5 mM; Mes buffer 20 mM; H2O 90%; D2O 10%

sample_3: vam7p, [U-15N], 0.5 mM; Mes buffer 20 mM; D2O 100%

sample_4: vam7p, [U-10% 13C], 0.5 mM; Mes buffer 20 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 20 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available

Software:

NMRPipe v1.8 - processing

VNMR v6.1 - collection

NMRView v4.1.2 - data analysis

CNS v1.0 - refinement

NMR spectrometers:

Varian INOVA 600 MHz

PDB	1KMD
GB	EGA86963