BMRB Entry 5365

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PDB ID: 2a3s
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5365
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C resonance assignments for the DNA-binding domain of myocyte nuclear factor

Deposition date:

2002-05-08

Original release date:

2003-01-13

Authors:

Chuang, Woei-Jer

Citation:

Citation: Chuang, Woei-Jer; Yeh, I-Ju; Hsieh, Yu-Huei; Liu, Pei-Phen; Chen, Shu-Wan; Jeng, W.-Y.. "Letters to the Editor: 1H, 15N, 13C resonance assignments for the DNA-binding domain of myocyte nuclear factor (Foxk1)" J. Biomol. NMR 24, 75-76 (2002).

Assembly members:

Assembly members:
myocyte nuclear factor, polymer, 114 residues, 13012 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
myocyte nuclear factor: ASMTGGQQMGRGSESKPPYS YAQLIVQAISSAQDRQLTLS GIYAHITKHYPYYRTADKGW QNSIRHNLSLNRYFIKVPRS QEEPGKGSFWRIDPASEAKL VEQAFRKRRQRGVS

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	661
13C chemical shifts	184
15N chemical shifts	89

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	myocyte nuclear factor	1

Entities:

Entity 1, myocyte nuclear factor 114 residues - 13012 Da.

1

ALA

SER

MET

THR

GLY

GLN

MET

GLY

2

ARG

GLY

SER

GLU

SER

LYS

PRO

TYR

SER

3

TYR

ALA

GLN

LEU

ILE

VAL

GLN

ALA

ILE

SER

4

SER

ALA

GLN

ASP

ARG

GLN

LEU

THR

LEU

SER

5

GLY

ILE

TYR

ALA

HIS

ILE

THR

LYS

HIS

TYR

6

PRO

TYR

ARG

THR

ALA

ASP

LYS

GLY

TRP

7

GLN

ASN

SER

ILE

ARG

HIS

ASN

LEU

SER

LEU

8

ASN

ARG

TYR

PHE

ILE

LYS

VAL

PRO

ARG

SER

9

GLN

GLU

PRO

GLY

LYS

GLY

SER

PHE

TRP

10

ARG

ILE

ASP

PRO

ALA

SER

GLU

ALA

LYS

LEU

11

VAL

GLU

GLN

ALA

PHE

ARG

LYS

ARG

GLN

12

ARG

GLY

VAL

SER

Samples:

sample_1: myocyte nuclear factor, [U-15N; U-13C], 3 mM; phosphate buffer 25 mM; NaCl 100 mM

sample_2: myocyte nuclear factor, [U-15N], 3 mM; phosphate buffer 25 mM; NaCl 100 mM

sample_3: myocyte nuclear factor, [15N]-Ala, 1 mM; phosphate buffer 25 mM; NaCl 100 mM

sample_4: myocyte nuclear factor 3 mM; phosphate buffer 25 mM; NaCl 100 mM

condition_1: pH: 6.0; temperature: 300 K; ionic strength: 1.5 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY-HSQC	not available	not available	not available
1H-15N TOCSY-HSQC	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCACB	not available	not available	not available
CACB(CO)NH	not available	not available	not available
HNHA	not available	not available	not available
HBHA(CBCA)NH	not available	not available	not available
HBHA(CBCACO)NH	not available	not available	not available
HCC(CO)NH-TOCSY	not available	not available	not available
HCCH-NOESY	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
HCCH-COSY	not available	not available	not available

Software:

aurelia v2.7.5 -

NMR spectrometers:

Bruker DRX 600 MHz

PDB	2A3S 2D2W
DBJ	BAE25880 BAE27871 BAG53651 BAG63595
GB	AAA37529 AAB69641 AAF97842 AAH54664 AAI56274
REF	NP_001032242 NP_001032296 NP_951031 NP_956196 XP_002723111
SP	P42128 P85037
TPG	DAA15159
AlphaFold	P42128 P85037 P42128 P85037