Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53625

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Glycosylated Fragment Crystallizable (Fc) Region of IgG1 Monoclonal Antibody
Published: 2026-03-23

Title:

Glycosylated Fragment Crystallizable (Fc) Region of IgG1 Monoclonal Antibody

Deposition date:

2026-03-12

Original release date:

2026-03-23

Authors:

Gagne, Donald; Hatfield, Grayson; Tepliakova, Lioudmila; Gingras, Genevieve; Stalker, Andrew; Li, Xuguang; Aubin, Yves; Tam, Roger

Citation:

Citation: Gagne, Donald; Aubin, Yves. "Complete and Rapid NMR Characterization of Therapeutic Monoclonal Antibodies via Differential Labelling of Fab fragments in Escherichia coli" Anal. Chem. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 224 residues, Formula weight is not available
entity_2, non-polymer, Formula weight is not available
entity_3, non-polymer, Formula weight is not available
entity_4, non-polymer, Formula weight is not available
entity_5, non-polymer, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: na Host organism: Pichia pastoris

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTCPPCPAPELLGGPSVFLF PPKPKDTLMISRTPEVTCVV VDVSHEDPEVKFNWYVDGVE VHNAKTKPREEQYNSTYRVV SVLTVLHQDWLNGKEYKCKV SNKALPAPIEKTISKAKGQP REPQVYTLPPSREEMTKNQV SLTCLVKGFYPSDIAVEWES NGQPENNYKTTPPVLDSDGS FFLYSKLTVDKSRWQQGNVF SCSVMHEALHNHYTQKSLSL SPGK

Data sets:

spectral_peak_list

Data type

Count

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	G0-FcEEM	1
2	G0-FcEEM	1
3	G1(6)-FcEEM	1
4	G1(6)-FcEEM	1
5	G1(3)-FcEEM	1
6	G1(3)-FcEEM	1
7	G2-FcEEM	1
8	G2-FcEEM	1

Entities:

Entity 1, G0-FcEEM 224 residues - Formula weight is not available

1

MET

THR

CYS

PRO

CYS

PRO

ALA

PRO

GLU

2

LEU

GLY

PRO

SER

VAL

PHE

LEU

PHE

3

PRO

LYS

PRO

LYS

ASP

THR

LEU

MET

ILE

4

SER

ARG

THR

PRO

GLU

VAL

THR

CYS

VAL

5

VAL

ASP

VAL

SER

HIS

GLU

ASP

PRO

GLU

VAL

6

LYS

PHE

ASN

TRP

TYR

VAL

ASP

GLY

VAL

GLU

7

VAL

HIS

ASN

ALA

LYS

THR

LYS

PRO

ARG

GLU

8

GLU

GLN

TYR

ASN

SER

THR

TYR

ARG

VAL

9

SER

VAL

LEU

THR

VAL

LEU

HIS

GLN

ASP

TRP

10

LEU

ASN

GLY

LYS

GLU

TYR

LYS

CYS

LYS

VAL

11

SER

ASN

LYS

ALA

LEU

PRO

ALA

PRO

ILE

GLU

12

LYS

THR

ILE

SER

LYS

ALA

LYS

GLY

GLN

PRO

13

ARG

GLU

PRO

GLN

VAL

TYR

THR

LEU

PRO

14

SER

ARG

GLU

MET

THR

LYS

ASN

GLN

VAL

15

SER

LEU

THR

CYS

LEU

VAL

LYS

GLY

PHE

TYR

16

PRO

SER

ASP

ILE

ALA

VAL

GLU

TRP

GLU

SER

17

ASN

GLY

GLN

PRO

GLU

ASN

TYR

LYS

THR

18

THR

PRO

VAL

LEU

ASP

SER

ASP

GLY

SER

19

PHE

LEU

TYR

SER

LYS

LEU

THR

VAL

ASP

20

LYS

SER

ARG

TRP

GLN

GLY

ASN

VAL

PHE

21

SER

CYS

SER

VAL

MET

HIS

GLU

ALA

LEU

HIS

22

ASN

HIS

TYR

THR

GLN

LYS

SER

LEU

SER

LEU

23

SER

PRO

GLY

LYS

Samples:

sample_1: FcEEM, [U-100% 13C; U-100% 15N], 15 uM; sodium acetate, [U-2H], 50 mM; G0 na na

sample_2: FcEEM, [U-100% 13C; U-100% 15N], 15 uM; sodium acetate, [U-2H], 50 uM; G1(6) na na

sample_3: FcEEM, [U-100% 13C; U-100% 15N], 15 uM; sodium acetate, [U-2H], 50 uM; G1(3) na na

sample_4: FcEEM, [U-100% 13C; U-100% 15N], 15 uM; sodium acetate, [U-2H], 50 uM; G2 na na

sample_conditions_1: pH: 6.0; temperature: 313 K

sample_conditions_2: pH: 6.0; temperature: 318 K

sample_conditions_3: pH: 6.0; temperature: 323 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_2
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_3
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_3
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_2
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_3
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_3
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_2
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_3
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_3
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_2
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_3
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_3

Software:

TOPSPIN - collection

NMRPipe - processing

NMRViewJ - data analysis

NMR spectrometers:

Bruker AVANCE III 700 MHz

BMRB Entry 53625