BMRB Entry 5362

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5362

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C Backbone resonance assignments of the 40 kDa LicT-CAT-PRD1 protein

Deposition date:

2002-05-03

Original release date:

2002-09-25

Authors:

Ducat, Thierry; Declerck, Nathalie; Kochoyan, Michel; Demene, Helene

Citation:

Citation: Ducat, Thierry; Declerck, Nathalie; Kochoyan, Michel; Demene, Helene. "Letter to the Editor: 1H, 15N and 13C Backbone resonance assignments of the 40 kDa LicT-CAT-PRD1 protein" J. Biomol. NMR 23, 325-326 (2002).

Assembly members:

Assembly members:
LicT CAT-PRD1, polymer, 175 residues, 19925.9 Da.

Natural source:

Natural source: Common Name: B. subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15 derivative

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LicT CAT-PRD1: MKIAKVINNNVISVVNEQGK ELVVMGRGLAFQKKSGDDVD EARIEKVFTLDNKDVSEKFK TLLYDIPIECMEVSEEIISY AKLQLGKKLNDSIYVSLTDH INFAIQRNQKGLDIKNALLW ETKRLYKDEFAIGKEALVMV KNKTGVSLPEDEAGFIALHI VNAELNELQHHHHHH

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	161
13C chemical shifts	481
15N chemical shifts	161

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CAT-PRD1 subunit 1	1
2	CAT-PRD1 subunit 2	1

Entities:

Entity 1, CAT-PRD1 subunit 1 175 residues - 19925.9 Da.

1

MET

LYS

ILE

ALA

LYS

VAL

ILE

ASN

2

VAL

ILE

SER

VAL

ASN

GLU

GLN

GLY

LYS

3

GLU

LEU

VAL

MET

GLY

ARG

GLY

LEU

ALA

4

PHE

GLN

LYS

SER

GLY

ASP

VAL

ASP

5

GLU

ALA

ARG

ILE

GLU

LYS

VAL

PHE

THR

LEU

6

ASP

ASN

LYS

ASP

VAL

SER

GLU

LYS

PHE

LYS

7

THR

LEU

TYR

ASP

ILE

PRO

ILE

GLU

CYS

8

MET

GLU

VAL

SER

GLU

ILE

SER

TYR

9

ALA

LYS

LEU

GLN

LEU

GLY

LYS

LEU

ASN

10

ASP

SER

ILE

TYR

VAL

SER

LEU

THR

ASP

HIS

11

ILE

ASN

PHE

ALA

ILE

GLN

ARG

ASN

GLN

LYS

12

GLY

LEU

ASP

ILE

LYS

ASN

ALA

LEU

TRP

13

GLU

THR

LYS

ARG

LEU

TYR

LYS

ASP

GLU

PHE

14

ALA

ILE

GLY

LYS

GLU

ALA

LEU

VAL

MET

VAL

15

LYS

ASN

LYS

THR

GLY

VAL

SER

LEU

PRO

GLU

16

ASP

GLU

ALA

GLY

PHE

ILE

ALA

LEU

HIS

ILE

17

VAL

ASN

ALA

GLU

LEU

ASN

GLU

LEU

GLN

HIS

18

HIS

Samples:

sample_1: LicT CAT-PRD1, [U-15N; U-13C; U-2H], 1.0 mM; Na Phosphate Buffer 10 mM; Na2SO4 50 mM; DTT 2 mM; EDTA 0.1 mM; Benzmidine 0.1 mM; H2O 90%; D2O 10%

condition_1: pH: 6.4 na; temperature: 293 K; ionic strength: 0.15 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N TROSY-HSQC	not available	not available	not available
TROSY-HNCA	not available	not available	not available
TROSY-HN(CO)CA	not available	not available	not available
TROSY-HNCACB	not available	not available	not available
TROSY-HN(CO)CACB	not available	not available	not available
TROSY-HNCO	not available	not available	not available
TROSY-HN(CA)CO	not available	not available	not available
15N edited-NOESY	not available	not available	not available

Software:

Gifa v4.4 - processing

NMRView v5.0 - assignment

NMR spectrometers:

Bruker DMX 600 MHz
Bruker DRX 500 MHz
Bruker DMX 800 MHz
Varian INOVA 800 MHz

PDB	1H99 1TLV
DBJ	BAA11696 BAI87589 BAM55992 BAM60004 GAK81310
EMBL	CAA82194 CAB15944 CCF07176 CCU57030 CEI59719
GB	ADM39914 ADV94733 AEP88876 AEP92976 AFI30559
REF	NP_391787 WP_003218864 WP_003236231 WP_003242598 WP_014115714
SP	P39805
AlphaFold	P39805