BMRB Entry 53605

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53605

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
1H, 15N chemical shift assignments for residues 2203-2284 of the FNIII12-14-V0-FNIII15 fragment of fibronectin
Deposition date:
2026-03-06
Original release date:
2026-11-01
Authors:
Banks, Paige; Blumson, Eve; Craig, Susan; Askari, Janet; Humphries, Jonathan; Tunnicliffe, Richard; Humphries, Martin; Waltho, Jonathan; Cliff, Matthew
Citation:

Citation: Banks, Paige; Blumson, Eve; Tunnicliffe, Richard; Craig, Sue; Askari, Janet; Humphries, Jonathan; Humphries, Martin; Waltho, Jonathan; Cliff, Matthew. "Structural characterisation of the IIICS-containing region of fibronectin: Splice variants define propensity for long-range interactions "  J. Struct. Biol. 218, 108379-108379 (2026).
PubMed: 42833290

Assembly members:

Assembly members:
entity_1, polymer, 353 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pFLAG-CTC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AIPAPTDLKFTQVTPTSLSA QWTPPNVQLTGYRVRVTPKE KTGPMKEINLAPDSSSVVVS GLMVATKYEVSVYALKDTLT SRPAQGVVTTLENVSPPRRA RVTDATETTITISWRTKTET ITGFQVDAVPANGQTPIQRT IKPDVRSYTITGLQPGTDYK IYLYTLNDNARSSPVVIDAS TAIDAPSNLRFLATTPNSLL VSWQPPRARITGYIIKYEKP GSPPREVVPRPRPGVTEATI TGLEPGTEYTIYVIALKNNQ KSEPLIGRKKTGQEALSQTT ISWAPFQDTSEYIISCHPVG TDEEPLQFRVPGTSTSATLT GLTRGATYNVIVEALKDQQR HKVREEVVTVGNS

Data sets:
Data typeCount
15N chemical shifts77
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
112-15FnIII fragment of fibronectin1

Entities:

Entity 1, 12-15FnIII fragment of fibronectin 353 residues - Formula weight is not available

1   ALAILEPROALAPROTHRASPLEULYSPHE
2   THRGLNVALTHRPROTHRSERLEUSERALA
3   GLNTRPTHRPROPROASNVALGLNLEUTHR
4   GLYTYRARGVALARGVALTHRPROLYSGLU
5   LYSTHRGLYPROMETLYSGLUILEASNLEU
6   ALAPROASPSERSERSERVALVALVALSER
7   GLYLEUMETVALALATHRLYSTYRGLUVAL
8   SERVALTYRALALEULYSASPTHRLEUTHR
9   SERARGPROALAGLNGLYVALVALTHRTHR
10   LEUGLUASNVALSERPROPROARGARGALA
11   ARGVALTHRASPALATHRGLUTHRTHRILE
12   THRILESERTRPARGTHRLYSTHRGLUTHR
13   ILETHRGLYPHEGLNVALASPALAVALPRO
14   ALAASNGLYGLNTHRPROILEGLNARGTHR
15   ILELYSPROASPVALARGSERTYRTHRILE
16   THRGLYLEUGLNPROGLYTHRASPTYRLYS
17   ILETYRLEUTYRTHRLEUASNASPASNALA
18   ARGSERSERPROVALVALILEASPALASER
19   THRALAILEASPALAPROSERASNLEUARG
20   PHELEUALATHRTHRPROASNSERLEULEU
21   VALSERTRPGLNPROPROARGALAARGILE
22   THRGLYTYRILEILELYSTYRGLULYSPRO
23   GLYSERPROPROARGGLUVALVALPROARG
24   PROARGPROGLYVALTHRGLUALATHRILE
25   THRGLYLEUGLUPROGLYTHRGLUTYRTHR
26   ILETYRVALILEALALEULYSASNASNGLN
27   LYSSERGLUPROLEUILEGLYARGLYSLYS
28   THRGLYGLNGLUALALEUSERGLNTHRTHR
29   ILESERTRPALAPROPHEGLNASPTHRSER
30   GLUTYRILEILESERCYSHISPROVALGLY
31   THRASPGLUGLUPROLEUGLNPHEARGVAL
32   PROGLYTHRSERTHRSERALATHRLEUTHR
33   GLYLEUTHRARGGLYALATHRTYRASNVAL
34   ILEVALGLUALALEULYSASPGLNGLNARG
35   HISLYSVALARGGLUGLUVALVALTHRVAL
36   GLYASNSER

Samples:

sample_1: 12-15FnIII fragment of fibronectin, [U-15N], 0.3 mM; MES 20 mM; NaCl 150 mM; EDTA 1 mM; sodium azide 0.02 % (w/v)

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

TOPSPIN - collection, processing

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks