BMRB Entry 5358

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5358

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Title:
1H, 15N, 13C Backbone Chemical Shift Assignments for Trypsin-Bound Bovine Pancreatic Trypsin Inhibitor (BPTI) at pH 5.8 and 36 Degrees
Deposition date:
2001-04-30
Original release date:
2002-05-08
Authors:
Biamonti, Clelia; Baran, Michael; Montelione, Gaetano
Citation:

Citation: Biamonti, Clelia. "Structural and Dynamic Investigations of Macromolecular Recognition Processes by Nuclear Magnetic Resonance Spectroscopy"  Thesis Rutgers University (1996).

Assembly members:

Assembly members:
bovine pancreatic trypsin inhibitor, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
bovine pancreatic trypsin inhibitor: RPDFCLEPPYTGPCKARIIR YFYNAKAGLCQTFVYGGCRA KRNNFKSAEDCMRTCGGA

Data sets:
Data typeCount
13C chemical shifts109
15N chemical shifts53
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BPTI1

Entities:

Entity 1, BPTI 58 residues - Formula weight is not available

1   ARGPROASPPHECYSLEUGLUPROPROTYR
2   THRGLYPROCYSLYSALAARGILEILEARG
3   TYRPHETYRASNALALYSALAGLYLEUCYS
4   GLNTHRPHEVALTYRGLYGLYCYSARGALA
5   LYSARGASNASNPHELYSSERALAGLUASP
6   CYSMETARGTHRCYSGLYGLYALA

Samples:

Sample_1: bovine pancreatic trypsin inhibitor 0.75 mM; CaCl2 25 mM; NaAzide 3 mM; H2O 90%; D2O 10%

BPTI_cond_1: pH: 5.8; temperature: 309 K

Experiments:

NameSampleSample stateSample conditions
HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
(HA)CANHnot availablenot availablenot available
(HA)CA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCACOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • VARIAN Unity 500 MHz

Related Database Links:

BMRB 1039 1156 1179 236 237 262 263 264 338 411 412 413 414 415 416 419 45 46 48 485 4868 49 4968 5171 5307 5359
PDB
EMBL CAA27062 CAA27063 CAA28371 CAA28886 CAA37967
GenBank AAA72535 AAB25189 AAD13685
PRF 1405218A 1405218D 1510193A 681071A
SWISS-PROT P00974

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks