BMRB Entry 53574
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53574
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Salgado, Gilmar. "Recognition and Resolution of KRAS 5'UTR RNA G-Quadruplexes by hnRNPA1" iScience ., .-..
Assembly members:
entity_1, polymer, 185 residues, Formula weight is not available
entity_2, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-UP1
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | protein (UP1) | 1 |
| 2 | RNA (UTR-1) | 2 |
Entities:
Entity 1, protein (UP1) 185 residues - Formula weight is not available
| 1 | GLY | PRO | LYS | GLU | PRO | GLU | GLN | LEU | ARG | LYS | ||||
| 2 | LEU | PHE | ILE | GLY | GLY | LEU | SER | PHE | GLU | THR | ||||
| 3 | THR | ASP | GLU | SER | LEU | ARG | SER | HIS | PHE | GLU | ||||
| 4 | GLN | TRP | GLY | THR | LEU | THR | ASP | CYS | VAL | VAL | ||||
| 5 | MET | ARG | ASP | PRO | ASN | THR | LYS | ARG | SER | ARG | ||||
| 6 | GLY | PHE | GLY | PHE | VAL | THR | TYR | ALA | THR | VAL | ||||
| 7 | GLU | GLU | VAL | ASP | ALA | ALA | MET | ASN | ALA | ARG | ||||
| 8 | PRO | HIS | LYS | VAL | ASP | GLY | ARG | VAL | VAL | GLU | ||||
| 9 | PRO | LYS | ARG | ALA | VAL | SER | ARG | GLU | ASP | SER | ||||
| 10 | GLN | ARG | PRO | GLY | ALA | HIS | LEU | THR | VAL | LYS | ||||
| 11 | LYS | ILE | PHE | VAL | GLY | GLY | ILE | LYS | GLU | ASP | ||||
| 12 | THR | GLU | GLU | HIS | HIS | LEU | ARG | ASP | TYR | PHE | ||||
| 13 | GLU | GLN | TYR | GLY | LYS | ILE | GLU | VAL | ILE | GLU | ||||
| 14 | ILE | MET | THR | ASP | ARG | GLY | SER | GLY | LYS | LYS | ||||
| 15 | ARG | GLY | PHE | ALA | PHE | VAL | THR | PHE | ASP | ASP | ||||
| 16 | HIS | ASP | SER | VAL | ASP | LYS | ILE | VAL | ILE | GLN | ||||
| 17 | LYS | TYR | HIS | THR | VAL | ASN | GLY | HIS | ASN | CYS | ||||
| 18 | GLU | VAL | ARG | LYS | ALA | LEU | SER | LYS | GLN | GLU | ||||
| 19 | MET | ALA | SER | ALA | SER |
Entity 2, RNA (UTR-1) 13 residues - Formula weight is not available
| 1 | A | G | G | U | G | G | C | G | G | C | ||||
| 2 | G | G | C |
Samples:
sample_1: protein (UP1), [U-98% 15N], 55 ± 0.1 uM; RNA (UTR-1), [U-98% 15N], 55 ± 0.1 uM; KPi 10 mM; KCl 50 mM
sample_conditions_1: ionic strength: 0.06 M; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.6 - data analysis
NMR spectrometers:
- Bruker AVANCE NEO 700 MHz