BMRB Entry 5355

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5355

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Title:
Backbone resonance assignments of the 25kD N-terminal ATPase domain from the Hsp90 chaperone
Deposition date:
2002-04-27
Original release date:
2002-09-25
Authors:
Williams, Mark; Salek, Reza; Ladbury, John
Citation:

Citation: Salek, Reza; Williams, Mark; Prodromou, Christosmos; Pearl, Laurence; Ladbury, John. "Letter to the Editor: Backbone resonance assignments of the 25kD N-terminal ATPase domain from the Hsp90 chaperone "  J. Biomol. NMR 23, 327-328 (2002).

Assembly members:

Assembly members:
Heat shock protein 90 N-terminal domain, polymer, 218 residues, 24709 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Heat shock protein 90 N-terminal domain: MRGSHHHHHHGMASETFEFQ AEITQLMSLIINTVYSNKEI FLRELISNASDALDKIRYKS LSDPKQLETEPDLFIRITPK PEQKVLEIRDSGIGMTKAEL INNLGTIAKSGTKAFMEALS AGADVSMIGQFGVGFYSLFL VADRVQVISKSNDDEQYIWE SNAGGSFTVTLDEVNERIGR GTILRLFLKDDQLEYLEEKR IKEVIKRHSEFVAYPIQL

Data sets:
Data typeCount
1H chemical shifts394
13C chemical shifts572
15N chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hsp90 N-terminal ATPase domain1

Entities:

Entity 1, Hsp90 N-terminal ATPase domain 218 residues - 24709 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYMETALASERGLUTHRPHEGLUPHEGLN
3   ALAGLUILETHRGLNLEUMETSERLEUILE
4   ILEASNTHRVALTYRSERASNLYSGLUILE
5   PHELEUARGGLULEUILESERASNALASER
6   ASPALALEUASPLYSILEARGTYRLYSSER
7   LEUSERASPPROLYSGLNLEUGLUTHRGLU
8   PROASPLEUPHEILEARGILETHRPROLYS
9   PROGLUGLNLYSVALLEUGLUILEARGASP
10   SERGLYILEGLYMETTHRLYSALAGLULEU
11   ILEASNASNLEUGLYTHRILEALALYSSER
12   GLYTHRLYSALAPHEMETGLUALALEUSER
13   ALAGLYALAASPVALSERMETILEGLYGLN
14   PHEGLYVALGLYPHETYRSERLEUPHELEU
15   VALALAASPARGVALGLNVALILESERLYS
16   SERASNASPASPGLUGLNTYRILETRPGLU
17   SERASNALAGLYGLYSERPHETHRVALTHR
18   LEUASPGLUVALASNGLUARGILEGLYARG
19   GLYTHRILELEUARGLEUPHELEULYSASP
20   ASPGLNLEUGLUTYRLEUGLUGLULYSARG
21   ILELYSGLUVALILELYSARGHISSERGLU
22   PHEVALALATYRPROILEGLNLEU

Samples:

sample_1: Heat shock protein 90 N-terminal domain, [U-98% 13C; U-98% 15N; U-70% 2H], 0.5 mM; Tris 20 mM

sample_2: Heat shock protein 90 N-terminal domain, [U-98% 15N], 0.5 mM; Tris 20.0 mM

Ex-cond_1: pH*: 8.0 na; temperature: 298 K; ionic strength: 0.0435 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CA)CBnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HN(COCA)CBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available

Software:

NmrPipe v2.1 - spectral processing

type_prob-seq_prob v1.0 - amino acid type determination, sequential assignment

ANSIG v3.3 - spectral visualization, analysis

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Varian UnityPlus 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ GAA26739
EMBL CAA91604 CAA97961 CAY86720
GB AAA02743 AHY77944 EDN60908 EDV10990 EDZ70041
REF NP_015084
SP P02829
TPG DAA11197
AlphaFold P02829

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks