Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53525

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Backbone assignment of TPPP/P25a full length
Published: 2026-05-21

Title:

Backbone assignment of TPPP/P25a full length

Deposition date:

2026-02-06

Original release date:

2026-05-21

Authors:

Zeng, Shuyi; zhang, Shenqing; zhang, shengnan; Fan, Yun; Xia, Wencheng; Chen, Feiyang; Huang, Cheng'an; Lv, Shiran; Lu, Jinxia; Sun, Yunpeng; Liu, Kai'en; Li, Yunxia; Zhang, Yaoyang; Wang, Jian; Liu, Cong; Li, Dan

Citation:

Citation: Zeng, Shuyi; Zhang, Shenqing; Zhang, Shengnan; Fan, Yun; Xia, Wencheng; Chen, Feiyang; Huang, Cheng'an; Lv, Shiran; Lu, Jinxia; Sun, Yunpeng; Liu, Kai'en; Li, Yunxia; Zhang, Yaoyang; Wang, Jian; Liu, Cong; Li, Dan. "TPPP/p25 amyloid seeding activity in cerebrospinal fluid as a specific diagnostic biomarker for multiple system atrophy" Cell ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 219 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MADKAKPAKAANRTPPKSPG DPSKDRAAKRLSLESEGAGE GAAASPELSALEEAFRRFAV HGDARATGREMHGKNWSKLC KDCQVIDGRNVTVTDVDIVF SKIKGKSCRTITFEQFQEAL EELAKKRFKDKSSEEAVREV HRLIEGKAPIISGVTKAISS PTVSRLTDTTKFTGSHKERF DPSGKGKGKAGRVDLVDESG YVSGYKHAGTYDQKVQGGK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	288
15N chemical shifts	133
1H chemical shifts	133

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TPPP/P25a full length	1

Entities:

Entity 1, TPPP/P25a full length 219 residues - Formula weight is not available

1

MET

ALA

ASP

LYS

ALA

LYS

PRO

ALA

LYS

ALA

2

ALA

ASN

ARG

THR

PRO

LYS

SER

PRO

GLY

3

ASP

PRO

SER

LYS

ASP

ARG

ALA

LYS

ARG

4

LEU

SER

LEU

GLU

SER

GLU

GLY

ALA

GLY

GLU

5

GLY

ALA

SER

PRO

GLU

LEU

SER

ALA

6

LEU

GLU

ALA

PHE

ARG

PHE

ALA

VAL

7

HIS

GLY

ASP

ALA

ARG

ALA

THR

GLY

ARG

GLU

8

MET

HIS

GLY

LYS

ASN

TRP

SER

LYS

LEU

CYS

9

LYS

ASP

CYS

GLN

VAL

ILE

ASP

GLY

ARG

ASN

10

VAL

THR

VAL

THR

ASP

VAL

ASP

ILE

VAL

PHE

11

SER

LYS

ILE

LYS

GLY

LYS

SER

CYS

ARG

THR

12

ILE

THR

PHE

GLU

GLN

PHE

GLN

GLU

ALA

LEU

13

GLU

LEU

ALA

LYS

ARG

PHE

LYS

ASP

14

LYS

SER

GLU

ALA

VAL

ARG

GLU

VAL

15

HIS

ARG

LEU

ILE

GLU

GLY

LYS

ALA

PRO

ILE

16

ILE

SER

GLY

VAL

THR

LYS

ALA

ILE

SER

17

PRO

THR

VAL

SER

ARG

LEU

THR

ASP

THR

18

LYS

PHE

THR

GLY

SER

HIS

LYS

GLU

ARG

PHE

19

ASP

PRO

SER

GLY

LYS

GLY

LYS

GLY

LYS

ALA

20

GLY

ARG

VAL

ASP

LEU

VAL

ASP

GLU

SER

GLY

21

TYR

VAL

SER

GLY

TYR

LYS

HIS

ALA

GLY

THR

22

TYR

ASP

GLN

LYS

VAL

GLN

GLY

LYS

Samples:

sample_1: TPPP/P25a full length, [U-98% 13C; U-98% 15N], 450 uM; sodium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

TOPSPIN - collection

NMRViewJ - chemical shift assignment

NMR spectrometers:

Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 53525