BMRB Entry 53508
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53508
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Fraser, Olivia; Schleicher, Madeline; Pagano, Maya; Showalter, Scott. "PAX3-FOXO1 Contacts BRD4 through Its Acetylated Intrinsically Disordered Region
" Biochemistry 65, 2066-2076 (2026).
PubMed: 42284071
Assembly members:
entity_1, polymer, 45 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-49b(+)
Entity Sequences (FASTA):
entity_1: VQNEGTGXSSWWMLNPEGGX
SGXSPRRRAASMDNNSKFAK
SRSRA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 116 |
| 15N chemical shifts | 45 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FOXO1-IDR-Acetyl | 1 |
Entities:
Entity 1, FOXO1-IDR-Acetyl 45 residues - Formula weight is not available
K233Ac, K245Ac, K248Ac
| 1 | VAL | GLN | ASN | GLU | GLY | THR | GLY | ALY | SER | SER | ||||
| 2 | TRP | TRP | MET | LEU | ASN | PRO | GLU | GLY | GLY | ALY | ||||
| 3 | SER | GLY | ALY | SER | PRO | ARG | ARG | ARG | ALA | ALA | ||||
| 4 | SER | MET | ASP | ASN | ASN | SER | LYS | PHE | ALA | LYS | ||||
| 5 | SER | ARG | SER | ARG | ALA |
Samples:
sample_1: FOXO1-IDR-Acetyl, [U-100% 13C; U-100% 15N], 1.2 mM; H2O 90%; D2O, [U-100% 2H], 10%; potassium phosphate 50 mM; potassium chloride 150 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 15N-13C CON-IPAP | sample_1 | isotropic | sample_conditions_1 |
| 2D 15N-13C CON-IPAP | sample_1 | isotropic | sample_conditions_1 |
| 3D (HACA)N(CA)CON | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCCON-IPAP | sample_1 | isotropic | sample_conditions_1 |
| 3D (HACA)N(CA)NCO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - processing
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
- Bruker AVANCE III 500 MHz