BMRB Entry 53485

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53485

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Title:
Cysteine-free N-domain of FAT10 bound to NUB1L
Deposition date:
2025-12-16
Original release date:
2026-05-29
Authors:
Weiss, Charlotte; Overall, Sarah; Catone, Nicola; Barnes, Alexander; Aichem, Annette; Groettrup, Marcus; Mathies, Guinevere
Citation:

Citation: Weiss, Charlotte; Overall, Sarah; Catone, Nicola; Barnes, Alexander; Aichem, Annette; Mathies, Guinevere. "Intermolecular beta-sheet Formation Guides the Interaction between Ubiquitin-like Modifier FAT10 and Adapter Protein NUB1L"  J. Am. Chem. Soc. ., .-. (2026).
PubMed: 42159595

Assembly members:

Assembly members:
entity_1, polymer, 83 residues, 9400 Da.
entity_2, polymer, 614 residues, 70400 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GASTLTVHVRSEEWDLMTFD ANPYDSVKKIKEHVRSKTKV PVQDQVLLLGSKILKPRRSL SSYGIDKEKTIHLTLKVVKP SDE
entity_2: AQKKYLQAKLTQFLREDRIQ LWKPPYTDENKKVGLALKDL AKQYSDRLECCENEVEKVIE EIRCKAIERGTGNDNYRTTG IATIEVFLPPRLKKDRKNLL ETRLHITGRELRSKIAETFG LQENYIKIVINKKQLQLGKT LEEQGVAHNVKAMVLELKQS EEDARKNFQLEEEEQNEAKL KEKQIQRTKRGLEILAKRAA ETVVDPEMTPYLDIANQTGR SIRIPPSERKALMLAMGYHE KGRAFLKRKEYGIALPCLLD ADKYFCECCRELLDTVDNYA VLQLDIVWCYFRLEQLECLD DAEKKLNLAQKCFKNCYGEN HQRLVHIKGNCGKEKVLFLR LYLLQGIRNYHSGNDVEAYE YLNKARQLFKELYIDPSKVD NLLQLGFTAQEARLGLRACD GNVDHAATHITNRREELAQI RKEEKEKKRRRLENIRFLKG MGYSTHAAQQVLHAASGNLD EALKILLSNPQMWWLNDSNP ETDNRQESPSQENIDRLVYM GFDALVAEAALRVFRGNVQL AAQTLAHNGGSLPPELPLSP EDSLSPPATSPSDSAGTSSA STDEDMETEAVNEILEDIPE HEEDYLDSTLEDEEIIIAEY LSYVENRKSATKKN

Data sets:
Data typeCount
13C chemical shifts79
15N chemical shifts19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 83 residues - 9400 Da.

N-terminal glycine residue results from TEV protease cleavage site.

1   GLYALASERTHRLEUTHRVALHISVALARG
2   SERGLUGLUTRPASPLEUMETTHRPHEASP
3   ALAASNPROTYRASPSERVALLYSLYSILE
4   LYSGLUHISVALARGSERLYSTHRLYSVAL
5   PROVALGLNASPGLNVALLEULEULEUGLY
6   SERLYSILELEULYSPROARGARGSERLEU
7   SERSERTYRGLYILEASPLYSGLULYSTHR
8   ILEHISLEUTHRLEULYSVALVALLYSPRO
9   SERASPGLU

Entity 2, entity_2 614 residues - 70400 Da.

1   ALAGLNLYSLYSTYRLEUGLNALALYSLEU
2   THRGLNPHELEUARGGLUASPARGILEGLN
3   LEUTRPLYSPROPROTYRTHRASPGLUASN
4   LYSLYSVALGLYLEUALALEULYSASPLEU
5   ALALYSGLNTYRSERASPARGLEUGLUCYS
6   CYSGLUASNGLUVALGLULYSVALILEGLU
7   GLUILEARGCYSLYSALAILEGLUARGGLY
8   THRGLYASNASPASNTYRARGTHRTHRGLY
9   ILEALATHRILEGLUVALPHELEUPROPRO
10   ARGLEULYSLYSASPARGLYSASNLEULEU
11   GLUTHRARGLEUHISILETHRGLYARGGLU
12   LEUARGSERLYSILEALAGLUTHRPHEGLY
13   LEUGLNGLUASNTYRILELYSILEVALILE
14   ASNLYSLYSGLNLEUGLNLEUGLYLYSTHR
15   LEUGLUGLUGLNGLYVALALAHISASNVAL
16   LYSALAMETVALLEUGLULEULYSGLNSER
17   GLUGLUASPALAARGLYSASNPHEGLNLEU
18   GLUGLUGLUGLUGLNASNGLUALALYSLEU
19   LYSGLULYSGLNILEGLNARGTHRLYSARG
20   GLYLEUGLUILELEUALALYSARGALAALA
21   GLUTHRVALVALASPPROGLUMETTHRPRO
22   TYRLEUASPILEALAASNGLNTHRGLYARG
23   SERILEARGILEPROPROSERGLUARGLYS
24   ALALEUMETLEUALAMETGLYTYRHISGLU
25   LYSGLYARGALAPHELEULYSARGLYSGLU
26   TYRGLYILEALALEUPROCYSLEULEUASP
27   ALAASPLYSTYRPHECYSGLUCYSCYSARG
28   GLULEULEUASPTHRVALASPASNTYRALA
29   VALLEUGLNLEUASPILEVALTRPCYSTYR
30   PHEARGLEUGLUGLNLEUGLUCYSLEUASP
31   ASPALAGLULYSLYSLEUASNLEUALAGLN
32   LYSCYSPHELYSASNCYSTYRGLYGLUASN
33   HISGLNARGLEUVALHISILELYSGLYASN
34   CYSGLYLYSGLULYSVALLEUPHELEUARG
35   LEUTYRLEULEUGLNGLYILEARGASNTYR
36   HISSERGLYASNASPVALGLUALATYRGLU
37   TYRLEUASNLYSALAARGGLNLEUPHELYS
38   GLULEUTYRILEASPPROSERLYSVALASP
39   ASNLEULEUGLNLEUGLYPHETHRALAGLN
40   GLUALAARGLEUGLYLEUARGALACYSASP
41   GLYASNVALASPHISALAALATHRHISILE
42   THRASNARGARGGLUGLULEUALAGLNILE
43   ARGLYSGLUGLULYSGLULYSLYSARGARG
44   ARGLEUGLUASNILEARGPHELEULYSGLY
45   METGLYTYRSERTHRHISALAALAGLNGLN
46   VALLEUHISALAALASERGLYASNLEUASP
47   GLUALALEULYSILELEULEUSERASNPRO
48   GLNMETTRPTRPLEUASNASPSERASNPRO
49   GLUTHRASPASNARGGLNGLUSERPROSER
50   GLNGLUASNILEASPARGLEUVALTYRMET
51   GLYPHEASPALALEUVALALAGLUALAALA
52   LEUARGVALPHEARGGLYASNVALGLNLEU
53   ALAALAGLNTHRLEUALAHISASNGLYGLY
54   SERLEUPROPROGLULEUPROLEUSERPRO
55   GLUASPSERLEUSERPROPROALATHRSER
56   PROSERASPSERALAGLYTHRSERSERALA
57   SERTHRASPGLUASPMETGLUTHRGLUALA
58   VALASNGLUILELEUGLUASPILEPROGLU
59   HISGLUGLUASPTYRLEUASPSERTHRLEU
60   GLUASPGLUGLUILEILEILEALAGLUTYR
61   LEUSERTYRVALGLUASNARGLYSSERALA
62   THRLYSLYSASN

Samples:

sample_1: N-FAT10-C0, [U-100% 13C; U-100% 15N], 1.5 mg/mL; NUB1L 10 mg/mL; HEPES 20 mM; sodium chloride 150 mM; TCEP 0.5 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1isotropicsample_conditions_1
2D 15N-13C ZF TEDORsample_1isotropicsample_conditions_1
3D 15N-13C-13C ZF TEDOR DARRsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 850 MHz