Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53484

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Cysteine-free N-domain of FAT10
Published: 2026-05-29

Title:

Cysteine-free N-domain of FAT10

Deposition date:

2025-12-16

Original release date:

2026-05-29

Authors:

Weiss, Charlotte; Overall, Sarah; Catone, Nicola; Barnes, Alexander; Aichem, Annette; Groettrup, Marcus; Mathies, Guinevere

Citation:

Citation: Weiss, Charlotte; Overall, Sarah; Catone, Nicola; Barnes, Alexander; Aichem, Annette; Mathies, Guinevere. "Intermolecular beta-sheet Formation Guides the Interaction between Ubiquitin-like Modifier FAT10 and Adapter Protein NUB1L" J. Am. Chem. Soc. ., .-. (2026).
PubMed: 42159595

Assembly members:

Assembly members:
entity_1, polymer, 83 residues, 9400 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GASTLTVHVRSEEWDLMTFD ANPYDSVKKIKEHVRSKTKV PVQDQVLLLGSKILKPRRSL SSYGIDKEKTIHLTLKVVKP SDE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	538
15N chemical shifts	135

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 83 residues - 9400 Da.

N-terminal glycine residue results from TEV protease cleavage site.

1

GLY

ALA

SER

THR

LEU

THR

VAL

HIS

VAL

ARG

2

SER

GLU

TRP

ASP

LEU

MET

THR

PHE

ASP

3

ALA

ASN

PRO

TYR

ASP

SER

VAL

LYS

ILE

4

LYS

GLU

HIS

VAL

ARG

SER

LYS

THR

LYS

VAL

5

PRO

VAL

GLN

ASP

GLN

VAL

LEU

GLY

6

SER

LYS

ILE

LEU

LYS

PRO

ARG

SER

LEU

7

SER

TYR

GLY

ILE

ASP

LYS

GLU

LYS

THR

8

ILE

HIS

LEU

THR

LEU

LYS

VAL

LYS

PRO

9

SER

ASP

GLU

Samples:

sample_1: N-FAT10-C0, [U-100% 13C; U-100% 15N], 10 mg/mL; HEPES 10 mM; sodium chloride 75 mM; ammonium sulfate 1.6 M; citric acid 50 mM

sample_2: N-FAT10-C0, [U-100% 13C; U-100% 15N], 8 mg/mL; HEPES 20 mM; sodium chloride 150 mM

sample_conditions_1: pH: 2.5; pressure: 1 atm; temperature: 277 K

sample_conditions_2: pH: 7.5; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C DARR	sample_1	isotropic	sample_conditions_1
2D 15N-13C ZF TEDOR	sample_1	isotropic	sample_conditions_1
3D 15N-13C-13C ZF TEDOR DARR	sample_1	isotropic	sample_conditions_1
2D 13C-13C DARR	sample_2	isotropic	sample_conditions_2
2D 15N-13C ZF TEDOR	sample_2	isotropic	sample_conditions_2
3D 15N-13C-13C ZF TEDOR DARR	sample_2	isotropic	sample_conditions_2
3D NCOCX	sample_2	isotropic	sample_conditions_2

Software:

TOPSPIN - collection, processing

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz
Bruker AVANCE III 850 MHz

BMRB Entry 53484