Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53483

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Backbone 1H, 13C and 15N Chemical Shift Assignments of N-Myc1-69
Published: 2026-01-29

Title:

Backbone 1H, 13C and 15N Chemical Shift Assignments of N-Myc1-69

Deposition date:

2025-12-16

Original release date:

2026-01-29

Authors:

Hultman, Johanna; Ahlner, Alexandra; Sunnerhagen, Maria

Citation:

Citation: Hultman, Johanna; Morad, Vivian; Tanner, Eliane; Kenney, Tristan; Pietras, Zuzanna; Pramod Khare, Lalit; Derbyshire, Dean; Resetca, Diana; Arrowsmith, Cheryl; Aili, Daniel; Ekstrom, Simon; Penn, Linda; Wallner, Bjorn; Ahlner, Alexandra; Sunnerhagen, Maria. "The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A" Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pNH-TrxT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPSCSTSTMPGMICKNPDLE FDSLQPCFYPDEDDFYFGGP DSTPPGEDIWKKFELLPTPP LSPSRGFAE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	193
15N chemical shifts	56
1H chemical shifts	216

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-Myc1-69	1

Entities:

Entity 1, N-Myc1-69 69 residues - Formula weight is not available

1

MET

PRO

SER

CYS

SER

THR

SER

THR

MET

PRO

2

GLY

MET

ILE

CYS

LYS

ASN

PRO

ASP

LEU

GLU

3

PHE

ASP

SER

LEU

GLN

PRO

CYS

PHE

TYR

PRO

4

ASP

GLU

ASP

PHE

TYR

PHE

GLY

PRO

5

ASP

SER

THR

PRO

GLY

GLU

ASP

ILE

TRP

6

LYS

PHE

GLU

LEU

PRO

THR

PRO

7

LEU

SER

PRO

SER

ARG

GLY

PHE

ALA

GLU

Samples:

sample_1: N-Myc1-69, [U-100% 13C; U-100% 15N], 164 uM; MES 20 mM; sodium chloride 100 mM; glycerol 5%; DTT 5 mM; TCEP 1 mM; sodium azide 100 uM; D2O, [U-2H], 10%

sample_conditions_1: pH: 6.5; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 53483