BMRB Entry 53482

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53482

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Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments of N-Myc1-100
Deposition date:
2025-12-16
Original release date:
2026-01-29
Authors:
Hultman, Johanna; Ahlner, Alexandra; Sunnerhagen, Maria
Citation:

Citation: Hultman, Johanna; Morad, Vivian; Tanner, Eliane; Kenney, Tristan; Pietras, Zuzanna; Pramod Khare, Lalit; Derbyshire, Dean; Resetca, Diana; Arrowsmith, Cheryl; Aili, Daniel; Ekstrom, Simon; Penn, Linda; Wallner, Bjorn; Ahlner, Alexandra; Sunnerhagen, Maria. "The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A"  Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNH-TrxT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPSCSTSTMPGMICKNPDLE FDSLQPCFYPDEDDFYFGGP DSTPPGEDIWKKFELLPTPP LSPSRGFAEHSSEPPSWVTE MLLENELWGSPAEEDAFGLG

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts84
1H chemical shifts315

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-Myc1-1001

Entities:

Entity 1, N-Myc1-100 100 residues - Formula weight is not available

1   METPROSERCYSSERTHRSERTHRMETPRO
2   GLYMETILECYSLYSASNPROASPLEUGLU
3   PHEASPSERLEUGLNPROCYSPHETYRPRO
4   ASPGLUASPASPPHETYRPHEGLYGLYPRO
5   ASPSERTHRPROPROGLYGLUASPILETRP
6   LYSLYSPHEGLULEULEUPROTHRPROPRO
7   LEUSERPROSERARGGLYPHEALAGLUHIS
8   SERSERGLUPROPROSERTRPVALTHRGLU
9   METLEULEUGLUASNGLULEUTRPGLYSER
10   PROALAGLUGLUASPALAPHEGLYLEUGLY

Samples:

sample_1: N-Myc1-100, [U-100% 13C; U-100% 15N], 98 uM; MES 20 mM; sodium chloride 100 mM; glycerol 5%; DTT 5 mM; TCEP 1 mM; sodium acetate 100 uM; D2O, [U-2H], 10%

sample_conditions_1: pH: 6.5; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks