BMRB Entry 53481
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53481
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Chen, Wen. "Ethylene-regulated dimeric ETR1 kinase reveals multi-site EIN2 docking targeted by peptides that delay climacteric ripening" Sci. Adv. ., .-..
Assembly members:
entity_1, polymer, 77 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PETM44
Entity Sequences (FASTA):
entity_1: STTAVTLLDLIKDVEMAISC
RKGRTGTAAGDVAFPKGKEN
LASVLKRYKRRLSNKPVGMN
QDGPGSRKNVTAYGSLG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 127 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 71 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EIN2-C | 1 |
Entities:
Entity 1, EIN2-C 77 residues - Formula weight is not available
| 1 | SER | THR | THR | ALA | VAL | THR | LEU | LEU | ASP | LEU | ||||
| 2 | ILE | LYS | ASP | VAL | GLU | MET | ALA | ILE | SER | CYS | ||||
| 3 | ARG | LYS | GLY | ARG | THR | GLY | THR | ALA | ALA | GLY | ||||
| 4 | ASP | VAL | ALA | PHE | PRO | LYS | GLY | LYS | GLU | ASN | ||||
| 5 | LEU | ALA | SER | VAL | LEU | LYS | ARG | TYR | LYS | ARG | ||||
| 6 | ARG | LEU | SER | ASN | LYS | PRO | VAL | GLY | MET | ASN | ||||
| 7 | GLN | ASP | GLY | PRO | GLY | SER | ARG | LYS | ASN | VAL | ||||
| 8 | THR | ALA | TYR | GLY | SER | LEU | GLY |
Samples:
sample_1: EIN2-C, [U-99% 15N], 0.5 mM
sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - data analysis
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks