BMRB Entry 53480
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53480
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Fanni, F.; Maruzs, B.; Kalman, Z.; Klumpler, T.; Batta, G.; Peterfia, B.; Gaspari, Z.. "Modulation of Homer1 EVH1 domain internal dynamics by putative autism-associated mutations" FEBS Lett. ., .-. (2026).
PubMed: 42206738
Assembly members:
entity_1, polymer, 121 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: pET-15b
Entity Sequences (FASTA):
entity_1: GSHMGEQPIFSTRAHVFQID
PNTKKNWVPTSKHAVTVSYF
YDSTRNVYRIISLDGSKAII
NSTITPNMTFTKTSQKFGQW
ADSRANTVYGLGFSSEHHLL
KFAEKFQEFKEAARLAKEKS
Q
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- order_parameters
| Data type | Count |
| 13C chemical shifts | 404 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 462 |
| T1 relaxation values | 85 |
| T2 relaxation values | 84 |
| heteronuclear NOE values | 79 |
| order parameters | 81 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Homer protein homolog 1 S97L mutant | 1 |
Entities:
Entity 1, Homer protein homolog 1 S97L mutant 121 residues - Formula weight is not available
The sequence contains an expression tag (GSH) not part of the natural protein.
| 1 | GLY | SER | HIS | MET | GLY | GLU | GLN | PRO | ILE | PHE | ||||
| 2 | SER | THR | ARG | ALA | HIS | VAL | PHE | GLN | ILE | ASP | ||||
| 3 | PRO | ASN | THR | LYS | LYS | ASN | TRP | VAL | PRO | THR | ||||
| 4 | SER | LYS | HIS | ALA | VAL | THR | VAL | SER | TYR | PHE | ||||
| 5 | TYR | ASP | SER | THR | ARG | ASN | VAL | TYR | ARG | ILE | ||||
| 6 | ILE | SER | LEU | ASP | GLY | SER | LYS | ALA | ILE | ILE | ||||
| 7 | ASN | SER | THR | ILE | THR | PRO | ASN | MET | THR | PHE | ||||
| 8 | THR | LYS | THR | SER | GLN | LYS | PHE | GLY | GLN | TRP | ||||
| 9 | ALA | ASP | SER | ARG | ALA | ASN | THR | VAL | TYR | GLY | ||||
| 10 | LEU | GLY | PHE | SER | SER | GLU | HIS | HIS | LEU | LEU | ||||
| 11 | LYS | PHE | ALA | GLU | LYS | PHE | GLN | GLU | PHE | LYS | ||||
| 12 | GLU | ALA | ALA | ARG | LEU | ALA | LYS | GLU | LYS | SER | ||||
| 13 | GLN |
Samples:
sample_1: Homer1 EVH1 S97L mutant, [U-13C; U-15N], 560 uM; Sodium phosphate buffer 50 mM; sodium chloride 20 mM; NaN3 0.02%
sample_2: Homer1 EVH1 S97L mutant, [U-15N], 228 uM; Sodium phosphate buffer 50 mM; sodium chloride 20 mM; NaN3 0.02%
sample_conditions_1: ionic strength: 215 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| T1/R1 relaxation | sample_2 | isotropic | sample_conditions_1 |
| T2/R2 relaxation | sample_2 | isotropic | sample_conditions_1 |
| 1H-15N heteronoe | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr Analysis v2.5.2 - peak picking
NMRFAM-SPARKY v1.413 - data analysis
Tensor v2 - data analysis
NMR spectrometers:
- Bruker AVANCE NEO 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks