BMRB Entry 53456

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53456

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Title:
CBP Nuclear Coactivator Binding Domain with a 4-(R) fluoroproline at position 2077
Deposition date:
2025-11-27
Original release date:
2026-06-02
Authors:
Kieffer, Bruno; Ben Bouzayene, Abir; Torbeev, Vladimir
Citation:

Citation: Ben Bouzayene, Abir; Sai, Mariam; Join, Alexis; Nomine, Yves; Torbeev, Vladimir; Kieffer, Bruno. "Distinct Molten-globule Conformations in an Interacting Protein Domain Resolved via 19F NMR of Fluoroprolines"  .

Assembly members:

Assembly members:
entity_1, polymer, 47 residues, 5273.1467 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SALQDLLRTLKSXSSPQQQQ QVLNILKSNPQLMAAFIKQR TAKYVAN

Data sets:
Data typeCount
13C chemical shifts141
15N chemical shifts26
1H chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MSM121

Entities:

Entity 1, MSM12 47 residues - 5273.1467 Da.

1   SERALALEUGLNASPLEULEUARGTHRLEU
2   LYSSERFP9SERSERPROGLNGLNGLNGLN
3   GLNVALLEUASNILELEULYSSERASNPRO
4   GLNLEUMETALAALAPHEILELYSGLNARG
5   THRALALYSTYRVALALAASN

Samples:

sample_1: MSM12 1 ± 0.1 mM; sodium phosphate 20 ± 1 mM

sample_2: MSM12 1 ± 0.1 mM; sodium phosphate 20 ± 1 mM

sample_conditions_1: ionic strength: 30 mM; pH: 6.8; pressure: 1 atm; temperature: 304 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC/HMQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2.4 - chemical shift assignment

DANGLE v1.1 - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

UniProt Q92793

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks