BMRB Entry 53447

Name: Hexim1 RNA-binding motif basic region BR
Published: 2025-12-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53447

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Hexim1 RNA-binding motif basic region BR

Deposition date:

2025-11-24

Original release date:

2025-12-16

Authors:

Yang, Yuan; Feigon, Juli

Citation:

Citation: Yang, Yuan; Murrali, Maria; Galvan, Sabrina; Wang, Yaqiang; Stephen, Christine; Ajjampore, Neha; Wang, Xiaoyu; Feigon, Juli. "HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation" Nat. Comm. ., .-. (2025).

Assembly members:

Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GWGQQQRQLGKKKHRRRPSK KKRHWK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	64
15N chemical shifts	22
1H chemical shifts	22

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hexim1 BR	1

Entities:

Entity 1, Hexim1 BR 26 residues - Formula weight is not available

Amino acids 141 through 165 from human Hexim1. A non-native glycine remains at the N-terminus of the final BR after TEV protease cleavage.

1

GLY

TRP

GLY

GLN

ARG

GLN

LEU

GLY

2

LYS

HIS

ARG

PRO

SER

LYS

3

LYS

ARG

HIS

TRP

LYS

Samples:

sample_1: sodium phosphate 50 mM; sodium azide 0.02 % w/v; D2O 5 % v/v; Hexim1 BR, [U-98% 13C; U-98% 15N], 0.73 mM; potassium chloride 150 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.2; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

istHMS - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz