BMRB Entry 53441

Name: HEXIM1 central region BR-L-AR protein assignments
Published: 2025-12-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53441

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HEXIM1 central region BR-L-AR protein assignments

Deposition date:

2025-11-24

Original release date:

2025-12-16

Authors:

Yang, Yuan; Feigon, Juli

Citation:

Citation: Yang, Yuan; Murrali, Maria; Galvan, Sabrina; Wang, Yaqiang; Stephen, Christine; Ajjampore, Neha; Wang, Xiaoyu; Feigon, Juli. "HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation" Nat. Comm. ., .-. (2025).

Assembly members:

Assembly members:
entity_1, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGQQQRQLGKKKHRRRPSKK KRHWKPYYKLTWEEKKKFDE KQSLRASRIRAEMFAKGQPV APYNTTQFLMDDHDQEEPDL KTGLYSKRAAAKSDDTSDDD FMEEGGEEDGGSD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	194
15N chemical shifts	102
1H chemical shifts	102

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BR-L-AR	1

Entities:

Entity 1, BR-L-AR 113 residues - Formula weight is not available

Amino acids 142 through 253 from human Hexim1. A non-native glycine remains at the N-terminus of the final full-length BR-L-AR after TEV protease cleavage.

1

GLY

GLN

ARG

GLN

LEU

GLY

LYS

2

LYS

HIS

ARG

PRO

SER

LYS

3

LYS

ARG

HIS

TRP

LYS

PRO

TYR

LYS

LEU

4

THR

TRP

GLU

LYS

PHE

ASP

GLU

5

LYS

GLN

SER

LEU

ARG

ALA

SER

ARG

ILE

ARG

6

ALA

GLU

MET

PHE

ALA

LYS

GLY

GLN

PRO

VAL

7

ALA

PRO

TYR

ASN

THR

GLN

PHE

LEU

MET

8

ASP

HIS

ASP

GLN

GLU

PRO

ASP

LEU

9

LYS

THR

GLY

LEU

TYR

SER

LYS

ARG

ALA

10

ALA

LYS

SER

ASP

THR

SER

ASP

11

PHE

MET

GLU

GLY

GLU

ASP

GLY

12

GLY

SER

ASP

Samples:

sample_1: Hexim1 BR-L-AR, [U-98% 13C; U-98% 15N], 0.07 mM; sodium phosphate 50 mM; sodium azide 0.02 % w/v; D2O 5 % v/v; potassium chloride 150 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.2; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

istHMS - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz