BMRB Entry 53439

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53439

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Title:
Chemical shifts of Dsk2 from S. cerevisiae
Deposition date:
2025-11-23
Original release date:
2025-12-26
Authors:
Acharya, Nirbhik; Castaneda, Carlos
Citation:

Citation: Acharya, Nirbhik; Daniel, Emily; Dao, Thuy; Niblo, Jessica; Mulvey, Erin; Sukenik, Shahar; Kraut, Daniel; Roelofs, Jeroen; Castaneda, Carlos. "STI1 domain dynamically engages transient helices in disordered regions to drive self-association and phase separation of yeast ubiquilin Dsk2"  EMBO J. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 373 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pE-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSLNIHIKSGQDKWEVNVAP ESTVLQFKEAINKANGIPVA NQRLIYSGKILKDDQTVESY HIQDGHSVHLVKSQPKPQTA SAAGANNATATGAAAGTGAT PNMSSGQSAGFNPLADLTSA RYAGYLNMPSADMFGPDGGA LNNDSNNQDELLRMMENPIF QSQMNEMLSNPQMLDFMIQS NPQLQAMGPQARQMLQSPMF RQMLTNPDMIRQSMQFARMM DPNAGMGSAGGAASAFPAPG GDAPEEGSNTNTTSSSNTGN NAGTNAGTNAGANTAANPFA SLLNPALNPFANAGNAASTG MPAFDPALLASMFQPPVQAS QAEDTRPPEERYEHQLRQLN DMGFFDFDRNVAALRRSGGS VQGALDSLLNGDV

Data sets:
Data typeCount
13C chemical shifts299
15N chemical shifts296
1H chemical shifts296

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dsk21

Entities:

Entity 1, Dsk2 373 residues - Formula weight is not available

1   METSERLEUASNILEHISILELYSSERGLY
2   GLNASPLYSTRPGLUVALASNVALALAPRO
3   GLUSERTHRVALLEUGLNPHELYSGLUALA
4   ILEASNLYSALAASNGLYILEPROVALALA
5   ASNGLNARGLEUILETYRSERGLYLYSILE
6   LEULYSASPASPGLNTHRVALGLUSERTYR
7   HISILEGLNASPGLYHISSERVALHISLEU
8   VALLYSSERGLNPROLYSPROGLNTHRALA
9   SERALAALAGLYALAASNASNALATHRALA
10   THRGLYALAALAALAGLYTHRGLYALATHR
11   PROASNMETSERSERGLYGLNSERALAGLY
12   PHEASNPROLEUALAASPLEUTHRSERALA
13   ARGTYRALAGLYTYRLEUASNMETPROSER
14   ALAASPMETPHEGLYPROASPGLYGLYALA
15   LEUASNASNASPSERASNASNGLNASPGLU
16   LEULEUARGMETMETGLUASNPROILEPHE
17   GLNSERGLNMETASNGLUMETLEUSERASN
18   PROGLNMETLEUASPPHEMETILEGLNSER
19   ASNPROGLNLEUGLNALAMETGLYPROGLN
20   ALAARGGLNMETLEUGLNSERPROMETPHE
21   ARGGLNMETLEUTHRASNPROASPMETILE
22   ARGGLNSERMETGLNPHEALAARGMETMET
23   ASPPROASNALAGLYMETGLYSERALAGLY
24   GLYALAALASERALAPHEPROALAPROGLY
25   GLYASPALAPROGLUGLUGLYSERASNTHR
26   ASNTHRTHRSERSERSERASNTHRGLYASN
27   ASNALAGLYTHRASNALAGLYTHRASNALA
28   GLYALAASNTHRALAALAASNPROPHEALA
29   SERLEULEUASNPROALALEUASNPROPHE
30   ALAASNALAGLYASNALAALASERTHRGLY
31   METPROALAPHEASPPROALALEULEUALA
32   SERMETPHEGLNPROPROVALGLNALASER
33   GLNALAGLUASPTHRARGPROPROGLUGLU
34   ARGTYRGLUHISGLNLEUARGGLNLEUASN
35   ASPMETGLYPHEPHEASPPHEASPARGASN
36   VALALAALALEUARGARGSERGLYGLYSER
37   VALGLNGLYALALEUASPSERLEULEUASN
38   GLYASPVAL

Samples:

sample_1: Dsk2, [U-99% 13C; U-99% 15N], 250 uM; sodium phosphate 20 mM; sodium azide 0.02%; EDTA 0.5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)Nsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

CcpNMR v2.5.1 - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P48510

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks