BMRB Entry 53438
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53438
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Suhler, Roni; van Beurden, Lars; Shmueli, Merav; Faust, Ofrah; Rosenzweig, Rina. "Human Hsp70 paralogs display selective J-domain interactions tuning proteostasis under stress
" Proc. Natl. Acad. Sci. U. S. A. 123, e2534903123-e2534903123 (2026).
PubMed: 42612003
Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b
Entity Sequences (FASTA):
entity_1: MGKDYYKILGIPSGANEDEI
KKAYRKMALKYHPDKNKEPN
AEEKFKEIAEAYDVLSDPKK
RGLYDQYGEEGLKTGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 217 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 70 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNAJB5 JD | 1 |
Entities:
Entity 1, DNAJB5 JD 76 residues - Formula weight is not available
| 1 | MET | GLY | LYS | ASP | TYR | TYR | LYS | ILE | LEU | GLY | ||||
| 2 | ILE | PRO | SER | GLY | ALA | ASN | GLU | ASP | GLU | ILE | ||||
| 3 | LYS | LYS | ALA | TYR | ARG | LYS | MET | ALA | LEU | LYS | ||||
| 4 | TYR | HIS | PRO | ASP | LYS | ASN | LYS | GLU | PRO | ASN | ||||
| 5 | ALA | GLU | GLU | LYS | PHE | LYS | GLU | ILE | ALA | GLU | ||||
| 6 | ALA | TYR | ASP | VAL | LEU | SER | ASP | PRO | LYS | LYS | ||||
| 7 | ARG | GLY | LEU | TYR | ASP | GLN | TYR | GLY | GLU | GLU | ||||
| 8 | GLY | LEU | LYS | THR | GLY | GLY |
Samples:
sample_1: DNAJB5 JD, [U-100% 13C; U-100% 15N], 3 mM; KCl 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v3 - chemical shift assignment
TOPSPIN - collection
NMRPipe - processing
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks