BMRB Entry 53427

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53427

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Title:
Backbone assignment of LARP6 NTR
Deposition date:
2025-11-18
Original release date:
2026-02-16
Authors:
Abis, Giancarlo; Simpson, Peter J; Capraro, Federica; Incocciati, Alessio; Kelly, Geoff; Mardakheh, Faraz; Conte, Maria R
Citation:

Citation: Abis, Giancarlo; Simpson, Peter J; Incocciati, Alessio; Capraro, Federica; Kelly, Geoff; Mardakheh, Faraz; Conte, Maria R. "An intrinsically disordered RNA-binding region provides local target selectivity and is essential for LARP6 function"  Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: in house modified pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAQSGGEARPGPKTAVQIRV AIQEAEDVDELEDEEEGAET RGAGDPARYLSPGWGSASEE EPSRGHSGTTASGGENERED LEQ

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts87
1H chemical shifts490

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LARP6 NTR1

Entities:

Entity 1, LARP6 NTR 83 residues - Formula weight is not available

1   METALAGLNSERGLYGLYGLUALAARGPRO
2   GLYPROLYSTHRALAVALGLNILEARGVAL
3   ALAILEGLNGLUALAGLUASPVALASPGLU
4   LEUGLUASPGLUGLUGLUGLYALAGLUTHR
5   ARGGLYALAGLYASPPROALAARGTYRLEU
6   SERPROGLYTRPGLYSERALASERGLUGLU
7   GLUPROSERARGGLYHISSERGLYTHRTHR
8   ALASERGLYGLYGLUASNGLUARGGLUASP
9   LEUGLUGLN

Samples:

sample_1: LARP6 NTR (M1-Q83), [U-100% 13C; U-100% 15N], 400 uM; TRIS 25 mM; KCl 100 mM; EDTA 0.2 mM; MgCl2 5 mM; TCEP 1 mM; Tween-20 0.1%

sample_conditions_1: ionic strength: 238 mM; pH: 7.25; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HBHA(CBCACO)NHsample_1isotropicsample_conditions_1
H(NCOCA)NNHsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1
(H)N(COCA)NNHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D 13C-detected (HCA)COCON-TOCSYsample_1isotropicsample_conditions_1
3D 13C-detected (H)CCCON-TOCSYsample_1isotropicsample_conditions_1
3D 13C-detected H(CC)CON-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR v3 - data analysis

MARS - chemical shift assignment

SMILE - data analysis

NEF-Pipelines - data analysis

NMR spectrometers:

  • Bruker AVANCE IIIHD 800 MHz
  • Bruker AVANCE IIIHD 700 MHz
  • Bruker AVANCE NEO 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks