BMRB Entry 53425

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53425

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Title:
mefp-12 -FP12 peptide
Deposition date:
2025-11-15
Original release date:
2025-12-16
Authors:
Rivard, Mathieu; Poulhazan, Alexandre; Renner-Rao, Max; Duthoo, Emilie; Jehle, Franziska; Flammang, Patrick; Jackson, Daniel; Harrington, Matthew
Citation:

Citation: Rivard, Mathieu; Poulhazan, Alexandre; Renner-Rao, Max; Duthoo, Emilie; Jehle, Franziska; Flammang, Patrick; Jackson, Daniel; Harrington, Matthew. "Histidine-rich coiled-coils promote zinc-dependent self-assembly and curing of porous mussel glues"  Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: blue mussel   Taxonomy ID: 6550   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mytilus edulis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: VDYHIKRLHKHTKYHLAAAE HQHKKHLNER

Data sets:
Data typeCount
13C chemical shifts119
15N chemical shifts30
1H chemical shifts254

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mefp-12 -FP121

Entities:

Entity 1, mefp-12 -FP12 30 residues - Formula weight is not available

acetylated sequence, synthesized using rink-amide resin

1   VALASPTYRHISILELYSARGLEUHISLYS
2   HISTHRLYSTYRHISLEUALAALAALAGLU
3   HISGLNHISLYSLYSHISLEUASNGLUARG

Samples:

sample_1: mefp-12 -FP12 5 mg/mL; H2O 90%; D2O, [U-100% 2H], 10%; DSS 5 uM; sodium chloride 1 M

sample_conditions_1: ionic strength: 1 M; pH: 4.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.9 - chemical shift assignment, collection, data analysis, peak picking, processing

MestReNova v16.0.0 - chemical shift assignment, data analysis, peak picking, processing

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks