BMRB Entry 5342
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5342
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Citation: ISERNIA, CARLA; BUCCI, ENRICO; LEONE, MARILISA; ZACCARO, LAURA; DI LELLO, PAOLA; DI GILIO, GIUSEPPE; ESPOSITO, SABRINA; SAVIANO, MICHELE; DI BLASIO, BENEDETTO; PEDONE, CARLO; PEDONE, PAOLO; FATTORUSSO, ROBERTO. "NMR Structure of the Single QALGGH Zinc Finger Domain from the Arabidopsis
thaliana SUPERMAN Protein" Chembiochem 4, 171-180 (2003).
PubMed: 12616630
Assembly members:
SUPERMAN 37, polymer, 37 residues, 4502.8 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
SUPERMAN 37: WPPRSYTCSFCKREFRSAQA
LGGHMNVHRRDRARLRL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 254 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SUPERMAN 37 | 1 |
| 2 | Zinc ion | 2 |
Entities:
Entity 1, SUPERMAN 37 37 residues - 4502.8 Da.
| 1 | TRP | PRO | PRO | ARG | SER | TYR | THR | CYS | SER | PHE | ||||
| 2 | CYS | LYS | ARG | GLU | PHE | ARG | SER | ALA | GLN | ALA | ||||
| 3 | LEU | GLY | GLY | HIS | MET | ASN | VAL | HIS | ARG | ARG | ||||
| 4 | ASP | ARG | ALA | ARG | LEU | ARG | LEU |
Entity 2, Zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
Sample_1: SUPERMAN 37 2 mM; ZINC (II) ION 2.3 mM
Experimental_Conditions: pH: 6.3; temperature: 301 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H DQFCOSY | Sample_1 | not available | Experimental_Conditions |
| 1H-1H TOCSY | Sample_1 | not available | Experimental_Conditions |
| 1H-1H NOESY | Sample_1 | not available | Experimental_Conditions |
| 1H-1H ROESY | Sample_1 | not available | Experimental_Conditions |
Software:
Varian Software (VNMR) vVNMR 6-1B - spectra processing
PROSA - spectra processing
XEASY - spectra analysis
NMR spectrometers:
- BRUKER AVANCE 600 MHz