BMRB Entry 53419

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53419

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Title:
The conserved region 2C of the human Forkhead box O4
Deposition date:
2025-11-05
Original release date:
2026-07-29
Authors:
Heo, Jeongbeen; Park, Chin-Ju
Citation:

Citation: Heo, Jeongbeen; Kim, Bohyeon; Park, Chin-Ju. "NMR investigation of conformational heterogeneity in FOXO4 CR2C binding to the MLL site of CBP KIX "  J. Magn. Reson. 391, 108136-108136 (2026).
PubMed: 42472485

Assembly members:

Assembly members:
entity_1, polymer, 31 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET His6 ProteinG TEV LIC cloning vector (1P)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: YLFSPAEGPLSAGEGCFSSS QALEALLTSDT

Data sets:
Data typeCount
13C chemical shifts84
15N chemical shifts29
1H chemical shifts29

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FOXO4 CR2C1

Entities:

Entity 1, FOXO4 CR2C 31 residues - Formula weight is not available

1   TYRLEUPHESERPROALAGLUGLYPROLEU
2   SERALAGLYGLUGLYCYSPHESERSERSER
3   GLNALALEUGLUALALEULEUTHRSERASP
4   THR

Samples:

sample_1: FOXO4 CR2C, [U-100% 13C; U-100% 15N], 550 uM; MES 20 mM; NaCl 100 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks