BMRB Entry 53413

Name: 1H, 15N, 13C backbone resonance assignment for S4E/S6E phosphomimetic double mutant of UP1
Published: 2025-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53413

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C backbone resonance assignment for S4E/S6E phosphomimetic double mutant of UP1

Deposition date:

2025-11-03

Original release date:

2025-11-10

Authors:

Das, Sayan; Venditti, Vincenzo

Citation:

Citation: Das, Sayan; Dunnett, Louise; Venditti, Vincenzo; Prischi, Filippo. "Phosphorylation-Dependent Remodeling of XIAP IRES by hnRNPA1" .

Assembly members:

Assembly members:
entity_1, polymer, 197 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-14

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGSKEEEPKEPEQLRKLFIG GLSFETTDESLRSHFEQWGT LTDCVVMRDPNTKRSRGFGF VTYATVEEVDAAMNARPHKV DGRVVEPKRAVSREDSQRPG AHLTVKKIFVGGIKEDTEEH HLRDYFEQYGKIEVIEIMTD RGSGKKRGFAFVTFDDHDSV DKIVIQKYHTVNGHNCEVRK ALSKQEMASASSSQRGR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	531
15N chemical shifts	178
1H chemical shifts	178

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UP1E	1

Entities:

Entity 1, UP1E 197 residues - Formula weight is not available

1

GLY

SER

LYS

GLU

PRO

LYS

GLU

2

PRO

GLU

GLN

LEU

ARG

LYS

LEU

PHE

ILE

GLY

3

GLY

LEU

SER

PHE

GLU

THR

ASP

GLU

SER

4

LEU

ARG

SER

HIS

PHE

GLU

GLN

TRP

GLY

THR

5

LEU

THR

ASP

CYS

VAL

MET

ARG

ASP

PRO

6

ASN

THR

LYS

ARG

SER

ARG

GLY

PHE

GLY

PHE

7

VAL

THR

TYR

ALA

THR

VAL

GLU

VAL

ASP

8

ALA

MET

ASN

ALA

ARG

PRO

HIS

LYS

VAL

9

ASP

GLY

ARG

VAL

GLU

PRO

LYS

ARG

ALA

10

VAL

SER

ARG

GLU

ASP

SER

GLN

ARG

PRO

GLY

11

ALA

HIS

LEU

THR

VAL

LYS

ILE

PHE

VAL

12

GLY

ILE

LYS

GLU

ASP

THR

GLU

HIS

13

HIS

LEU

ARG

ASP

TYR

PHE

GLU

GLN

TYR

GLY

14

LYS

ILE

GLU

VAL

ILE

GLU

ILE

MET

THR

ASP

15

ARG

GLY

SER

GLY

LYS

ARG

GLY

PHE

ALA

16

PHE

VAL

THR

PHE

ASP

HIS

ASP

SER

VAL

17

ASP

LYS

ILE

VAL

ILE

GLN

LYS

TYR

HIS

THR

18

VAL

ASN

GLY

HIS

ASN

CYS

GLU

VAL

ARG

LYS

19

ALA

LEU

SER

LYS

GLN

GLU

MET

ALA

SER

ALA

20

SER

GLN

ARG

GLY

ARG

Samples:

sample_1: UP1E, [U-13C; U-15N; U-1H], 1 mM; sodium phosphate 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

MARS - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks