BMRB Entry 5341

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5341

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Title:
1H, 13C, and 15N backbone assignment of an extended N-terminal, DNA-binding domain construct of the replication initiation protein from a geminivirus
Deposition date:
2002-04-09
Original release date:
2002-11-04
Authors:
Campos-Olivas, Ramon; Louis, John; Gronenborn, Bruno; Gronenborn, Angela
Citation:

Citation: Campos-Olivas, Ramon; Louis, John; Clerot, D.; Gronenborn, Bruno; Gronenborn, Angela. "1H, 13C, and 15N assignment of the N-terminal, catalytic domain of the replication initiation protein from the geminivirus TYLCV"  J. Biomol. NMR 24, 73-74 (2002).

Assembly members:

Assembly members:
N-terminal DNA-binding domain of Rep protein from a geminivirus, polymer, 159 residues, 17922.92 Da.

Natural source:

Natural source:   Common Name: Tomato yellow leaf curl virus - Sardinia   Taxonomy ID: 123735   Superkingdom: Viruses   Kingdom: not available   Genus/species: Begomovirus Tomato yellow leaf curl virus - Sardinia

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-terminal DNA-binding domain of Rep protein from a geminivirus: MRGSHHHHHHGIPGSGSGDD DDKMPRSGRFSIKAKNYFLT YPKCDLTKENALSQITNLQT PTNKLFIKICRELHENGEPH LHILIQFEGKYNCTNQRFFD LVSPTRSAHFHPNIQGAKSS SDVKSYIDKDGDVLEWGTFQ IDGRSARGGQQTANDAYAK

Data sets:
Data typeCount
1H chemical shifts172
13C chemical shifts290
15N chemical shifts156

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NTD-Rep, H6_rep1-1361

Entities:

Entity 1, NTD-Rep, H6_rep1-136 159 residues - 17922.92 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYILEPROGLYSERGLYSERGLYASPASP
3   ASPASPLYSMETPROARGSERGLYARGPHE
4   SERILELYSALALYSASNTYRPHELEUTHR
5   TYRPROLYSCYSASPLEUTHRLYSGLUASN
6   ALALEUSERGLNILETHRASNLEUGLNTHR
7   PROTHRASNLYSLEUPHEILELYSILECYS
8   ARGGLULEUHISGLUASNGLYGLUPROHIS
9   LEUHISILELEUILEGLNPHEGLUGLYLYS
10   TYRASNCYSTHRASNGLNARGPHEPHEASP
11   LEUVALSERPROTHRARGSERALAHISPHE
12   HISPROASNILEGLNGLYALALYSSERSER
13   SERASPVALLYSSERTYRILEASPLYSASP
14   GLYASPVALLEUGLUTRPGLYTHRPHEGLN
15   ILEASPGLYARGSERALAARGGLYGLYGLN
16   GLNTHRALAASNASPALATYRALALYS

Samples:

sample1: N-terminal DNA-binding domain of Rep protein from a geminivirus, [10%-13C; U-99% 15N], 0.6 – 0.8 mM; sodium phosphate 20 mM; NaCl 300 mM; DTT 1 mM; D2O 8%; H2O 92%

sample2: N-terminal DNA-binding domain of Rep protein from a geminivirus, [U-13C; U-99% 15N], 0.6 – 0.8 mM; sodium phosphate 20 mM; NaCl 300 mM; DTT 1 mM; D2O 8%; H2O 92%

sample3: N-terminal DNA-binding domain of Rep protein from a geminivirus, [U-10% 13C; U-99% 15N], 0.6 – 0.8 mM; sodium phosphate 20 mM; NaCl 300 mM; DTT 1 mM; D2O 100%

sample4: N-terminal DNA-binding domain of Rep protein from a geminivirus, [U-13C; U-99% 15N], 0.6 – 0.8 mM; sodium phosphate 20 mM; NaCl 300 mM; DTT 1 mM; D2O 100%

cond_H2O: pH: 6.6; temperature: 298 K; ionic strength: 1.0 M

cond_D2O: pH*: 6.6; temperature: 298 K; ionic strength: 1.0 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQC/HMQCnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HNCOCAnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CC(CO)NHnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D 15N-edited NOESYnot availablenot availablenot available
4D 13C,13C NOESYnot availablenot availablenot available

Software:

nmrpipe - processing

nmrview v4.1.1 - assignment

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 5297
PDB
EMBL CAA43466
GB ACE02956 ACE02974 ADF43040 AFQ00071
REF NP_620741
SP P27260
AlphaFold P27260

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks