BMRB Entry 53407

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53407

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Title:
Tau AT8
Deposition date:
2025-10-27
Original release date:
2026-03-30
Authors:
Boll, Emmanuelle; Cantrelle, Francois-xavier; Smet-Nocca, Caroline
Citation:

Citation: Boll, Emmanuelle; Cantrelle, Francois-xavier; Smet-Nocca, Caroline. "Combining experiments and simulations to study the impact of multiple phosphorylations at the AT8 epitope of the Tau protein"  PLOS One ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 441 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAEPRQEFEVMEDHAGTYGL GDRKDQGGYTMHQDQEGDTD AGLKEAPLQAPTEDGSEEPG SETSDAKSAPTAEDVTAPLV DEGAPGKQAAAQPHTEIPEG TTAEEAGIGDTPSLEDEAAG HVTQARMVSKSKDGTGSDDK KAKGADGKTKIAAPRGAAPP GQKGQANATRIPAKAPPAPK APPSSGEPPKAGDRSGYSAP GSPGTPGSRSRTPSLPAPPT REPKKVAVVRAPPKAPSSAK SRLQTAPVPMPDLKNVKSKI GATENLKHQPGGGKVQIINK KLDLSNVQSKCGSKDNIKHV PGGGSVQIVYKPVDLSKVTS KCGSLGNIHHKPGGGQVEVK SEKLDFKDRVQSKIGALDNI THVPGGGNKKIETHKLTFRE NAKAKTDHGAEIVYKAPVVS GDTAPRHLSNVSSTGSIDMV DAPQLATLADEVSASLAKQG L

Data sets:
Data typeCount
13C chemical shifts1028
15N chemical shifts373
1H chemical shifts343

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tau-AT81

Entities:

Entity 1, Tau-AT8 441 residues - Formula weight is not available

1   METALAGLUPROARGGLNGLUPHEGLUVAL
2   METGLUASPHISALAGLYTHRTYRGLYLEU
3   GLYASPARGLYSASPGLNGLYGLYTYRTHR
4   METHISGLNASPGLNGLUGLYASPTHRASP
5   ALAGLYLEULYSGLUALAPROLEUGLNALA
6   PROTHRGLUASPGLYSERGLUGLUPROGLY
7   SERGLUTHRSERASPALALYSSERALAPRO
8   THRALAGLUASPVALTHRALAPROLEUVAL
9   ASPGLUGLYALAPROGLYLYSGLNALAALA
10   ALAGLNPROHISTHRGLUILEPROGLUGLY
11   THRTHRALAGLUGLUALAGLYILEGLYASP
12   THRPROSERLEUGLUASPGLUALAALAGLY
13   HISVALTHRGLNALAARGMETVALSERLYS
14   SERLYSASPGLYTHRGLYSERASPASPLYS
15   LYSALALYSGLYALAASPGLYLYSTHRLYS
16   ILEALAALAPROARGGLYALAALAPROPRO
17   GLYGLNLYSGLYGLNALAASNALATHRARG
18   ILEPROALALYSALAPROPROALAPROLYS
19   ALAPROPROSERSERGLYGLUPROPROLYS
20   ALAGLYASPARGSERGLYTYRSERALAPRO
21   GLYSERPROGLYTHRPROGLYSERARGSER
22   ARGTHRPROSERLEUPROALAPROPROTHR
23   ARGGLUPROLYSLYSVALALAVALVALARG
24   ALAPROPROLYSALAPROSERSERALALYS
25   SERARGLEUGLNTHRALAPROVALPROMET
26   PROASPLEULYSASNVALLYSSERLYSILE
27   GLYALATHRGLUASNLEULYSHISGLNPRO
28   GLYGLYGLYLYSVALGLNILEILEASNLYS
29   LYSLEUASPLEUSERASNVALGLNSERLYS
30   CYSGLYSERLYSASPASNILELYSHISVAL
31   PROGLYGLYGLYSERVALGLNILEVALTYR
32   LYSPROVALASPLEUSERLYSVALTHRSER
33   LYSCYSGLYSERLEUGLYASNILEHISHIS
34   LYSPROGLYGLYGLYGLNVALGLUVALLYS
35   SERGLULYSLEUASPPHELYSASPARGVAL
36   GLNSERLYSILEGLYALALEUASPASNILE
37   THRHISVALPROGLYGLYGLYASNLYSLYS
38   ILEGLUTHRHISLYSLEUTHRPHEARGGLU
39   ASNALALYSALALYSTHRASPHISGLYALA
40   GLUILEVALTYRLYSALAPROVALVALSER
41   GLYASPTHRALAPROARGHISLEUSERASN
42   VALSERSERTHRGLYSERILEASPMETVAL
43   ASPALAPROGLNLEUALATHRLEUALAASP
44   GLUVALSERALASERLEUALALYSGLNGLY
45   LEU

Samples:

sample_1: Tau-AT8, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 25 mM; sodium chloride 50 mM; EDTA 2.5 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.099 M; pH: 6.4; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1D 15Nsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
hNcaNNHsample_1isotropicsample_conditions_1
HncaNNHsample_1isotropicsample_conditions_1
n-CaCONsample_1isotropicsample_conditions_1
NCOsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.5.2 - chemical shift assignment, peak picking

TOPSPIN v4.4.0 - collection

MddNMR v2.7 - processing

NMR spectrometers:

  • Bruker AVANCE NEO 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks