BMRB Entry 53355

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53355

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Title:
NMR assignment of the CrsL and CrsL-CarD complex from Mycobacterium smegmatis
Deposition date:
2025-09-17
Original release date:
2025-09-20
Authors:
Cerny, Martin; Shoman, Mahmoud; Hnilicova, Jarmila; Zidek, Lukas
Citation:

Citation: Shoman, Mahmoud; Jirat Matejckova, Jitka; Schwarz, Marek; Cerny, Martin; Borah, Nabajyoti; Vankova Hausnerova, Viola; Sikova, Michaela; Sanderova, Hana; Halada, Petr; Hubalek, Martin; Dvorakova, Vera; Prevorovsky, Martin; Holubova, Jana; Stanek, Ondrej; Krasny, Libor; Zidek, Lukas; Hnilicova, Jarmila. "Expanding the CarD interaction network: CrsL is a novel transcription factor in Mycobacterium smegmatis"  Nucleic Acids Res. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 62 residues, 5970 Da.
entity_2, polymer, 163 residues, 17991.44 Da.

Natural source:

Natural source:   Common Name: Mycobacterium smegmatis   Taxonomy ID: 1772   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium smegmatis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPAKTDPADLGDVEPLADDT ASQARRVVATYATDADECRM FLSMLGIGPSKTEVLEHHHH HH
entity_2: GMIFKVGDTVVYPHHGAALI EAIETRTIKGEQKEYLVLKV AQGDLTVRVPADNAEYVGVR DVVGQEGLDKVFQVLRAPHT EEPTNWSRRYKANLEKLASG DVNKVAEVVRDLWRRDQERG LSAGEKRMLAKARQILVGEL ALAENTDDAKAETILDEVLA AAS

Data sets:
Data typeCount
13C chemical shifts69
15N chemical shifts43
1H chemical shifts59

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CrsL1
2CarD2

Entities:

Entity 1, CrsL 62 residues - 5970 Da.

1   METPROALALYSTHRASPPROALAASPLEU
2   GLYASPVALGLUPROLEUALAASPASPTHR
3   ALASERGLNALAARGARGVALVALALATHR
4   TYRALATHRASPALAASPGLUCYSARGMET
5   PHELEUSERMETLEUGLYILEGLYPROSER
6   LYSTHRGLUVALLEUGLUHISHISHISHIS
7   HISHIS

Entity 2, CarD 163 residues - 17991.44 Da.

1   GLYMETILEPHELYSVALGLYASPTHRVAL
2   VALTYRPROHISHISGLYALAALALEUILE
3   GLUALAILEGLUTHRARGTHRILELYSGLY
4   GLUGLNLYSGLUTYRLEUVALLEULYSVAL
5   ALAGLNGLYASPLEUTHRVALARGVALPRO
6   ALAASPASNALAGLUTYRVALGLYVALARG
7   ASPVALVALGLYGLNGLUGLYLEUASPLYS
8   VALPHEGLNVALLEUARGALAPROHISTHR
9   GLUGLUPROTHRASNTRPSERARGARGTYR
10   LYSALAASNLEUGLULYSLEUALASERGLY
11   ASPVALASNLYSVALALAGLUVALVALARG
12   ASPLEUTRPARGARGASPGLNGLUARGGLY
13   LEUSERALAGLYGLULYSARGMETLEUALA
14   LYSALAARGGLNILELEUVALGLYGLULEU
15   ALALEUALAGLUASNTHRASPASPALALYS
16   ALAGLUTHRILELEUASPGLUVALLEUALA
17   ALAALASER

Samples:

sample_1: CrsL, [U-100% 13C; U-100% 15N], 0.4 mM; sodium phosphate 20 mM; sodium chloride 10 mM; sodium azide 1 mM; TCEP 0.5 mM

sample_2: CrsL, [U-100% 13C; U-100% 15N], 0.4 mM; TRIS 50 mM; sodium chloride 300 mM; sodium azide 1 mM; TCEP 0.5 mM; CarD 0.8 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 300.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v1.470 - chemical shift assignment, peak picking

TOPSPIN v4.0.9, 4.1.4 - collection

NMRDraw v11.2 - processing

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE NEO 950 MHz
  • Bruker AVANCE NEO 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks