BMRB Entry 5335

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PDB ID: 1lv3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5335
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side chain 1H, 13C, and 15N chemical shift assignments for E.coli protein YacG

Deposition date:

2002-03-27

Original release date:

2002-09-09

Authors:

Ramelot, Theresa; Kennedy, Michael

Citation:

Citation: Ramelot, Theresa; Cort, J.; Yee, A.; Semesi, A.; Edwards, A.; Arrowsmith, C.; Kennedy, Michael. "NMR Structure of the Eschericia coli Protein YacG: A Novel Sequence Motif in the Zinc-finger Family of Proteins " Proteins 49, 289-293 (2002).
PubMed: 12211008

Assembly members:

Assembly members:
YacG, polymer, 68 residues, 7306 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Eschericia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Eschericia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YacG: GSHMSETITVNCPTCGKTVV WGEISPFRPFCSKRCQLIDL GEWAAEEKRIPSSGDLSESD DWSEEPKQ

Data sets:

assigned_chemical_shifts
- YacG_shifts

Data type	Count
13C chemical shifts	256
1H chemical shifts	385
15N chemical shifts	61

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	YacG	1
2	zinc ion	2

Entities:

Entity 1, YacG 68 residues - 7306 Da.

1

GLY

SER

HIS

MET

SER

GLU

THR

ILE

THR

VAL

2

ASN

CYS

PRO

THR

CYS

GLY

LYS

THR

VAL

3

TRP

GLY

GLU

ILE

SER

PRO

PHE

ARG

PRO

PHE

4

CYS

SER

LYS

ARG

CYS

GLN

LEU

ILE

ASP

LEU

5

GLY

GLU

TRP

ALA

GLU

LYS

ARG

ILE

6

PRO

SER

GLY

ASP

LEU

SER

GLU

SER

ASP

7

ASP

TRP

SER

GLU

PRO

LYS

GLN

Entity 2, zinc ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: YacG, [U-13C; U-15N], 1 mM; phosphate buffer 25 mM; NaCl 450 mM

sample_2: YacG, [U-15N], 1 – 3 mM; phosphate buffer 25 mM; NaCl 450 mM

cond_1: pH: 6.5 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	cond_1
1H-13C HSQC (aliph)	not available	not available	cond_1
15N-edited NOESY-HQSC	not available	not available	cond_1
HCC-TOCSY-NNH	not available	not available	cond_1
HCCH-TOCSY	not available	not available	cond_1
CN-NOESY-HSQC	not available	not available	cond_1
HNCO	not available	not available	cond_1
HNCACB	not available	not available	cond_1
CBCA(CO)NH	not available	not available	cond_1
CCC-TOCSY-NNH	not available	not available	cond_1
HNHA	not available	not available	cond_1
4D CC-NOESY	not available	not available	cond_1
1H-13C HSQC (alpha)	not available	not available	cond_1
1H-13C HSQC (arom)	not available	not available	cond_1

Software:

Felix v98 - processing data

XPLOR v3.84 - structure calculations

NMR spectrometers:

Varian Inova 600 MHz
Varian Inova 750 MHz
Varian Inova 800 MHz

SWISS-PROT	P0A8H8 P0A8H9 A7ZW52 B1IR78 A7ZHJ2
REF	NP_706056 NP_308132 NP_414643 AP_000763 NP_285797
GenBank	AAN78619 AAP15644 AAG54405 AAN41763 AAC73212
DBJ	BAB96668 BAB33528
PDB	1LV3