BMRB Entry 5334

Title:
Backbone 1H, 13C, and 15N resonance assignments and secondary structure of the D54A mutant of HTLV-I capsid protein
Deposition date:
2002-03-26
Original release date:
2003-06-10
Authors:
Cornilescu, Claudia; Bouamr, Fadila; Carter, Carol; Tjandra, Nico
Citation:

Citation: Cornilescu, Claudia; Bouamr, Fadila; Carter, Carol; Tjandra, Nico. "Backbone 1H, 13C, and 15N resonance assignments and secondary structure of the D54A mutant of HTLV-I capsid protein "  .

Assembly members:

Assembly members:
Human T-cell Leukemia Virus type I, polymer, 134 residues, 14841.8 Da.

Natural source:

Natural source:   Common Name: Human T-cell lymphotropic virus type 1   Taxonomy ID: 11908   Superkingdom: Viruses   Kingdom: not available   Genus/species: Deltaretrovirus HTLV-I

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts436
13C chemical shifts361
15N chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HTLV-I1

Entities:

Entity 1, HTLV-I 134 residues - 14841.8 Da.

1   PROVALMETHISPROHISGLYALAPROPRO
2   ASNHISARGPROTRPGLNMETLYSASPLEU
3   GLNALAILELYSGLNGLUVALSERGLNALA
4   ALAPROGLYSERPROGLNPHEMETGLNTHR
5   ILEARGLEUALAVALGLNGLNPHEASPPRO
6   THRALALYSALALEUGLNASPLEULEUGLN
7   TYRLEUCYSSERSERLEUVALALASERLEU
8   HISHISGLNGLNLEUASPSERLEUILESER
9   GLUALAGLUTHRARGGLYILETHRSERTYR
10   ASNPROLEUALAGLYPROLEUARGVALGLN
11   ALAASNASNPROGLNGLNGLNGLYLEUARG
12   ARGGLUTYRGLNGLNLEUTRPLEUALAALA
13   PHEALAALALEUPROGLYSERALALYSASP
14   PROSERTRPALA

Samples:

N15_sample: Human T-cell Leukemia Virus type I, [U-15N], 0.8 – 1.0 mM

C13-N15_sample: Human T-cell Leukemia Virus type I, [U-13C; U-15N], 0.8 – 1.0 mM

sample_conditions_1: pH: 6.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablesample_conditions_1
CBCACONHnot availablenot availablesample_conditions_1
HNCACBnot availablenot availablesample_conditions_1
HBHACONHnot availablenot availablesample_conditions_1
3D HNCOnot availablenot availablesample_conditions_1
3D 1H-15N NOESYnot availablenot availablesample_conditions_1
1H-13C CT-HSQCnot availablenot availablesample_conditions_1

Software:

NMRPipe - processed data

TALOS - predicted torsion angles

PIPP_STAPP - peak assignments

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 4649
PDB
DBJ BAA02929 BAF31288 BAH85785 BAP28584
EMBL CAA34075 CAA62992
GB AAA85325 AAA85841 AAA96672 AAB20767 AAC00185
REF NP_049558 NP_057860 NP_057861 NP_057862 NP_955618
SP P03345 P03362 P10274 P14074 P14076
AlphaFold P14076 P03345 P03362 P10274 P14074

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks