BMRB Entry 53339

Name: 1H 13C 15N assignment of IDR3-domain (362-419) of nucleocapsid protein of SARS-CoV 2 in the full-length protein
Published: 2025-11-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53339

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H 13C 15N assignment of IDR3-domain (362-419) of nucleocapsid protein of SARS-CoV 2 in the full-length protein

Deposition date:

2025-09-02

Original release date:

2025-11-11

Authors:

Bolognesi, Tessa; Schiavina, Marco; Ciabini, Cristina; Pierattelli, Roberta; Felli, Isabella

Citation:

Citation: Bolognesi, Tessa; Schiavina, Marco; Ciabini, Cristina; Parafioriti, Michela; Gardini, Cristina; Elli, Stefano; Guerrini, Marco; Pierattelli, Roberta; Felli, Isabella. "Exploring the Role of Structural and Dynamic Complexity in SARS-CoV-2 Nucleocapsid Protein-Heparin Interactions by NMR" J. Mol. Biol. 437, 169437-169437 (2025).
PubMed: 40945576

Assembly members:

Assembly members:
entity_1, polymer, 419 residues, 45620 Da.

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet29b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDNGPQNQRNAPRITFGGP SDSTGSNQNGERSGARSKQR RPQGLPNNTASWFTALTQHG KEDLKFPRGQGVPINTNSSP DDQIGYYRRATRRIRGGDGK MKDLSPRWYFYYLGTGPEAG LPYGANKDGIIWVATEGALN TPKDHIGTRNPANNAAIVLQ LPQGTTLPKGFYAEGSRGGS QASSRSSSRSRNSSRNSTPG SSRGTSPARMAGNGGDAALA LLLLDRLNQLESKMSGKGQQ QQGQTVTKKSAAEASKKPRQ KRTATKAYNVTQAFGRRGPE QTQGNFGDQELIRQGTDYKH WPQIAQFAPSASAFFGMSRI GMEVTPSGTWLTYTGAIKLD DKDPNFKDQVILLNKHIDAY KTFPPTEPKKDKKKKADETQ ALPQRQKKQQTVTLLPAADL DDFSKQLQQSMSSADSTQA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	141
15N chemical shifts	49
1H chemical shifts	44

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nucleocapsid protein of SARS-CoV 2	1

Entities:

Entity 1, Nucleocapsid protein of SARS-CoV 2 419 residues - 45620 Da.

1

MET

SER

ASP

ASN

GLY

PRO

GLN

ASN

GLN

ARG

2

ASN

ALA

PRO

ARG

ILE

THR

PHE

GLY

PRO

3

SER

ASP

SER

THR

GLY

SER

ASN

GLN

ASN

GLY

4

GLU

ARG

SER

GLY

ALA

ARG

SER

LYS

GLN

ARG

5

ARG

PRO

GLN

GLY

LEU

PRO

ASN

THR

ALA

6

SER

TRP

PHE

THR

ALA

LEU

THR

GLN

HIS

GLY

7

LYS

GLU

ASP

LEU

LYS

PHE

PRO

ARG

GLY

GLN

8

GLY

VAL

PRO

ILE

ASN

THR

ASN

SER

PRO

9

ASP

GLN

ILE

GLY

TYR

ARG

ALA

10

THR

ARG

ILE

ARG

GLY

ASP

GLY

LYS

11

MET

LYS

ASP

LEU

SER

PRO

ARG

TRP

TYR

PHE

12

TYR

LEU

GLY

THR

GLY

PRO

GLU

ALA

GLY

13

LEU

PRO

TYR

GLY

ALA

ASN

LYS

ASP

GLY

ILE

14

ILE

TRP

VAL

ALA

THR

GLU

GLY

ALA

LEU

ASN

15

THR

PRO

LYS

ASP

HIS

ILE

GLY

THR

ARG

ASN

16

PRO

ALA

ASN

ALA

ILE

VAL

LEU

GLN

17

LEU

PRO

GLN

GLY

THR

LEU

PRO

LYS

GLY

18

PHE

TYR

ALA

GLU

GLY

SER

ARG

GLY

SER

19

GLN

ALA

SER

ARG

SER

ARG

SER

20

ARG

ASN

SER

ARG

ASN

SER

THR

PRO

GLY

21

SER

ARG

GLY

THR

SER

PRO

ALA

ARG

MET

22

ALA

GLY

ASN

GLY

ASP

ALA

LEU

ALA

23

LEU

ASP

ARG

LEU

ASN

GLN

LEU

24

GLU

SER

LYS

MET

SER

GLY

LYS

GLY

GLN

25

GLN

GLY

GLN

THR

VAL

THR

LYS

SER

26

ALA

GLU

ALA

SER

LYS

PRO

ARG

GLN

27

LYS

ARG

THR

ALA

THR

LYS

ALA

TYR

ASN

VAL

28

THR

GLN

ALA

PHE

GLY

ARG

GLY

PRO

GLU

29

GLN

THR

GLN

GLY

ASN

PHE

GLY

ASP

GLN

GLU

30

LEU

ILE

ARG

GLN

GLY

THR

ASP

TYR

LYS

HIS

31

TRP

PRO

GLN

ILE

ALA

GLN

PHE

ALA

PRO

SER

32

ALA

SER

ALA

PHE

GLY

MET

SER

ARG

ILE

33

GLY

MET

GLU

VAL

THR

PRO

SER

GLY

THR

TRP

34

LEU

THR

TYR

THR

GLY

ALA

ILE

LYS

LEU

ASP

35

ASP

LYS

ASP

PRO

ASN

PHE

LYS

ASP

GLN

VAL

36

ILE

LEU

ASN

LYS

HIS

ILE

ASP

ALA

TYR

37

LYS

THR

PHE

PRO

THR

GLU

PRO

LYS

38

ASP

LYS

ALA

ASP

GLU

THR

GLN

39

ALA

LEU

PRO

GLN

ARG

GLN

LYS

GLN

40

THR

VAL

THR

LEU

PRO

ALA

ASP

LEU

41

ASP

PHE

SER

LYS

GLN

LEU

GLN

SER

42

MET

SER

ALA

ASP

SER

THR

GLN

ALA

Samples:

sample_1: Nucleocapsid protein of SARS-CoV 2, [U-100% 13C; U-100% 15N], 100 uM; TRIS 25 mM; sodium chloride 450 mM; sodium azide 0.05%; EDTA 20 uM

sample_conditions_1: ionic strength: 0.45 M; pH: 7.3; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N BEST TROSY	sample_1	isotropic	sample_conditions_1
2D 13C-15N CON	sample_1	isotropic	sample_conditions_1
2D 13C-13C hCACO	sample_1	isotropic	sample_conditions_1
2D 13C-13C hCBCACO	sample_1	isotropic	sample_conditions_1
3D BEST TROSY HNCO	sample_1	isotropic	sample_conditions_1
3D BEST TROSY HNCACO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HCBCACON	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

XEASY - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE NEO 1200 MHz
Bruker AVANCE NEO 700 MHz
Bruker AVANCE NEO 700 MHz