BMRB Entry 53318

Name: Backbone chemical shift assignment for S. cerevisiae Tom20 cytosolic domain
Published: 2025-08-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53318

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignment for S. cerevisiae Tom20 cytosolic domain

Deposition date:

2025-08-14

Original release date:

2025-08-19

Authors:

Sucec, Iva; Schanda, Paul

Citation:

Citation: Sucec, Iva; Schanda, Paul. "Dynamic disorder orchestrates the mitochondrial protein import gate" .

Assembly members:

Assembly members:
entity_1, polymer, 152 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-GB1-TEV-stop

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMPQFRKVLRQRAKEQAKM EEQAKTHAKEVKLQKVTEFL SMELAKDPIPSDPSEREATF TTNVENGERLSMQQGKELEA ASKFYKALTVYPQPADLLGI YQRSIPEAIYEYIILMIAIL PPANVASFVKGVVGSKAESD AVAEANDIDDLE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	184
15N chemical shifts	67
1H chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ScTom20(37-183)	1

Entities:

Entity 1, ScTom20(37-183) 152 residues - Formula weight is not available

1

GLY

HIS

MET

PRO

GLN

PHE

ARG

LYS

VAL

LEU

2

ARG

GLN

ARG

ALA

LYS

GLU

GLN

ALA

LYS

MET

3

GLU

GLN

ALA

LYS

THR

HIS

ALA

LYS

GLU

4

VAL

LYS

LEU

GLN

LYS

VAL

THR

GLU

PHE

LEU

5

SER

MET

GLU

LEU

ALA

LYS

ASP

PRO

ILE

PRO

6

SER

ASP

PRO

SER

GLU

ARG

GLU

ALA

THR

PHE

7

THR

ASN

VAL

GLU

ASN

GLY

GLU

ARG

LEU

8

SER

MET

GLN

GLY

LYS

GLU

LEU

GLU

ALA

9

ALA

SER

LYS

PHE

TYR

LYS

ALA

LEU

THR

VAL

10

TYR

PRO

GLN

PRO

ALA

ASP

LEU

GLY

ILE

11

TYR

GLN

ARG

SER

ILE

PRO

GLU

ALA

ILE

TYR

12

GLU

TYR

ILE

LEU

MET

ILE

ALA

ILE

LEU

13

PRO

ALA

ASN

VAL

ALA

SER

PHE

VAL

LYS

14

GLY

VAL

GLY

SER

LYS

ALA

GLU

SER

ASP

15

ALA

VAL

ALA

GLU

ALA

ASN

ASP

ILE

ASP

16

LEU

GLU

Samples:

sample_1: Tom20(37-183), [U-13C; U-15N; U-2H], 0.55 mM; sodium chloride 100 mM; TRIS 20 mM

sample_conditions_1: ionic strength: 0.109 M; pH: 7; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA (BEST TROSY)	sample_1	isotropic	sample_conditions_1
3D HNCO (BEST TROSY)	sample_1	isotropic	sample_conditions_1
3D HNCOCA (BEST TROSY)	sample_1	isotropic	sample_conditions_1
3D HNCACB (BEST TROSY)	sample_1	isotropic	sample_conditions_1
3D HNCOCACB (BEST TROSY)	sample_1	isotropic	sample_conditions_1
3D HNCACO (BEST TROSY)	sample_1	isotropic	sample_conditions_1
2D HN BEST TROSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

CcpNMR vv3 - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE III 850 MHz