BMRB Entry 53303

Name: Amide backbone assignment of DNAJB1 J-domain
Published: 2025-11-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53303

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Amide backbone assignment of DNAJB1 J-domain

Deposition date:

2025-08-07

Original release date:

2025-11-06

Authors:

Leder, Anna; Mas, Guillaume; Hiller, Sebastian

Citation:

Citation: Leder, Anna; Mas, Guillaume; Hiller, Sebastian. "A universal tyrosine-based regulatory switch controls J-domain protein activity" Nat. Comm. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCA528

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGKDYYQTLGLARGASDEEI KRAYRRQALRYHPDKNKEPG AEEKFKEIAEAYDVLSDPRK REIFDRYGEEGLKGSGC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	60
1H chemical shifts	60

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNAJB1 J-domain	1

Entities:

Entity 1, DNAJB1 J-domain 77 residues - Formula weight is not available

1

MET

GLY

LYS

ASP

TYR

GLN

THR

LEU

GLY

2

LEU

ALA

ARG

GLY

ALA

SER

ASP

GLU

ILE

3

LYS

ARG

ALA

TYR

ARG

GLN

ALA

LEU

ARG

4

TYR

HIS

PRO

ASP

LYS

ASN

LYS

GLU

PRO

GLY

5

ALA

GLU

LYS

PHE

LYS

GLU

ILE

ALA

GLU

6

ALA

TYR

ASP

VAL

LEU

SER

ASP

PRO

ARG

LYS

7

ARG

GLU

ILE

PHE

ASP

ARG

TYR

GLY

GLU

8

GLY

LEU

LYS

GLY

SER

GLY

CYS

Samples:

sample_1: DNAJB1 J-domain, [U-100% 15N], 1 mM; MES 25 mM; potassium chloride 150 mM; magensium chloride 10 mM; DTT 5 mM; DSS 0.5 mM; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.5.2 - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks