BMRB Entry 53294

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53294

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Title:
Streptococcus pneumoniae HtrA PD domain
Deposition date:
2025-07-30
Original release date:
2026-01-23
Authors:
Lee, Eunjeong
Citation:

Citation: Lee, Eunjeong; Redzic, Jasmina; Gordon, Blaine; Saviola, Anthony; Tran, Norman; Maroney, Sean; Ashby, Nathanael; Shaw, Steven; Fulte, Sam; McCarty, Arianna; Holyoak, Todd; Meyer, Nancy; Hansen, Kirk; Clark, Sarah; Eisenmesser, Elan. "Streptococcus pneumoniae HtrA is a dynamic and monomeric virulence factor capable of forming larger oligomeric complexes"  Protein Sci. 35, e70411-e70411 (2026).
PubMed: 41457497

Assembly members:

Assembly members:
entity_1, polymer, 226 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: STTQAVNKVKDAVVSVITYS ANRQNSVFGNDDTDTDSQRI SSEGSGVIYKKNDKEAYIVT NNHVINGASKVDIRLSDGTK VPGEIVGADTFSDIAVVKIS SEKVTTVAEFGDSSKLTVGE TAIAIGSPLGSEYANTVTQG IVSSLNRNVSLKSEDGQAIS TKAIQTDTAINPGNSGGPLI NIQGQVIGITSSKIATNGGT SVEGLGFAIPANDAINIIEQ LEKNGK

Data sets:
Data typeCount
13C chemical shifts414
15N chemical shifts219
1H chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HtrA PD1

Entities:

Entity 1, HtrA PD 226 residues - Formula weight is not available

1   SERTHRTHRGLNALAVALASNLYSVALLYS
2   ASPALAVALVALSERVALILETHRTYRSER
3   ALAASNARGGLNASNSERVALPHEGLYASN
4   ASPASPTHRASPTHRASPSERGLNARGILE
5   SERSERGLUGLYSERGLYVALILETYRLYS
6   LYSASNASPLYSGLUALATYRILEVALTHR
7   ASNASNHISVALILEASNGLYALASERLYS
8   VALASPILEARGLEUSERASPGLYTHRLYS
9   VALPROGLYGLUILEVALGLYALAASPTHR
10   PHESERASPILEALAVALVALLYSILESER
11   SERGLULYSVALTHRTHRVALALAGLUPHE
12   GLYASPSERSERLYSLEUTHRVALGLYGLU
13   THRALAILEALAILEGLYSERPROLEUGLY
14   SERGLUTYRALAASNTHRVALTHRGLNGLY
15   ILEVALSERSERLEUASNARGASNVALSER
16   LEULYSSERGLUASPGLYGLNALAILESER
17   THRLYSALAILEGLNTHRASPTHRALAILE
18   ASNPROGLYASNSERGLYGLYPROLEUILE
19   ASNILEGLNGLYGLNVALILEGLYILETHR
20   SERSERLYSILEALATHRASNGLYGLYTHR
21   SERVALGLUGLYLEUGLYPHEALAILEPRO
22   ALAASNASPALAILEASNILEILEGLUGLN
23   LEUGLULYSASNGLYLYS

Samples:

sample_1: HtrA PD mM; D2O, [U-2H], 10%; sodium chloride 50 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks