BMRB Entry 53282
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53282
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Elmaleh, Bar; Faust, Ofrah; Rosenzweig, Rina. "The ALS-associated E425K mutation uncouples DNAJC7 from the Hsp70 chaperone cycle" FEBS J. ., .-. (2026).
PubMed: 41531269
Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b
Entity Sequences (FASTA):
entity_1: DYYKILGVDKNASEDEIKKA
YRKRALMHHPDRHSGASAEV
QKEEEKKFKEVGEAFTILSD
PKKKTRYDSGQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 199 |
| 15N chemical shifts | 68 |
| 1H chemical shifts | 68 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNAJC7_JD | 1 |
Entities:
Entity 1, DNAJC7_JD 71 residues - Formula weight is not available
| 1 | ASP | TYR | TYR | LYS | ILE | LEU | GLY | VAL | ASP | LYS | ||||
| 2 | ASN | ALA | SER | GLU | ASP | GLU | ILE | LYS | LYS | ALA | ||||
| 3 | TYR | ARG | LYS | ARG | ALA | LEU | MET | HIS | HIS | PRO | ||||
| 4 | ASP | ARG | HIS | SER | GLY | ALA | SER | ALA | GLU | VAL | ||||
| 5 | GLN | LYS | GLU | GLU | GLU | LYS | LYS | PHE | LYS | GLU | ||||
| 6 | VAL | GLY | GLU | ALA | PHE | THR | ILE | LEU | SER | ASP | ||||
| 7 | PRO | LYS | LYS | LYS | THR | ARG | TYR | ASP | SER | GLY | ||||
| 8 | GLN |
Samples:
sample_1: DNAJC7_JD, [U-100% 13C; U-100% 15N], 3 mM
sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
TOPSPIN - collection
NMRPipe - processing
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks