BMRB Entry 53266

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53266

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Title:
primase-DNA-2bp
Deposition date:
2025-07-10
Original release date:
2026-03-18
Authors:
Wu, Pengzhi; Senning, Niklas; Allain, Frederic
Citation:

Citation: Wu, Pengzhi; Damberger, Fred; Zehnder, Johannes; Schroder, Nina; Senning, Niklas; Lipps, Georg; Wiegand, Thomas; Allain, Frederic. "Molecular Basis for Primer Synthesis Initiation by DNA Primase"  .

Assembly members:

Assembly members:
entity_1, polymer, 331 residues, Formula weight is not available
entity_2, polymer, 9 residues, Formula weight is not available
entity_ATP, non-polymer, 507.181 Da.
entity_DGT, non-polymer, 507.181 Da.
entity_MG, non-polymer, 24.305 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Saccharolobus islandicus   Taxonomy ID: 43080   Superkingdom: not available   Kingdom: Thermoproteati   Genus/species: Saccharolobus islandicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SSERIRYAKWFLEHGFNIIP IDPESKKPVLKEWQKYSHEM PSDEEKQRFLKMIEEGYNYA IPGGQKGLVILDFESKEKLK AWIGESALEELCRKTLCTNT VHGGIHIYVLSNDIPPHKIN PLFEENGKGIIDLQSYNSYV LGLGSCVNHLHCTTDKCPWK EQNYTTCYTLYNELKEISKV DLKSLLRFLAEKGKRLGITL SKTAKEWLEGKKEEEDTVVE FEELRKELVKRDSGKPVEKI KEEICTKSPPKLIKEIICEN KTYADVNIDRSRGDWHVILY LMKHGVTDPDKILELLPRDS KAKENEKWNTQKYFVITLSK AWSVVKKYLEA
entity_2: CTGTGCTCA

Data sets:
Data typeCount
15N chemical shifts256
1H chemical shifts256

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2DNA2
3ATP3
4dGTP4
5MG5
6ZN6

Entities:

Entity 1, protein 331 residues - Formula weight is not available

1   SERSERGLUARGILEARGTYRALALYSTRP
2   PHELEUGLUHISGLYPHEASNILEILEPRO
3   ILEASPPROGLUSERLYSLYSPROVALLEU
4   LYSGLUTRPGLNLYSTYRSERHISGLUMET
5   PROSERASPGLUGLULYSGLNARGPHELEU
6   LYSMETILEGLUGLUGLYTYRASNTYRALA
7   ILEPROGLYGLYGLNLYSGLYLEUVALILE
8   LEUASPPHEGLUSERLYSGLULYSLEULYS
9   ALATRPILEGLYGLUSERALALEUGLUGLU
10   LEUCYSARGLYSTHRLEUCYSTHRASNTHR
11   VALHISGLYGLYILEHISILETYRVALLEU
12   SERASNASPILEPROPROHISLYSILEASN
13   PROLEUPHEGLUGLUASNGLYLYSGLYILE
14   ILEASPLEUGLNSERTYRASNSERTYRVAL
15   LEUGLYLEUGLYSERCYSVALASNHISLEU
16   HISCYSTHRTHRASPLYSCYSPROTRPLYS
17   GLUGLNASNTYRTHRTHRCYSTYRTHRLEU
18   TYRASNGLULEULYSGLUILESERLYSVAL
19   ASPLEULYSSERLEULEUARGPHELEUALA
20   GLULYSGLYLYSARGLEUGLYILETHRLEU
21   SERLYSTHRALALYSGLUTRPLEUGLUGLY
22   LYSLYSGLUGLUGLUASPTHRVALVALGLU
23   PHEGLUGLULEUARGLYSGLULEUVALLYS
24   ARGASPSERGLYLYSPROVALGLULYSILE
25   LYSGLUGLUILECYSTHRLYSSERPROPRO
26   LYSLEUILELYSGLUILEILECYSGLUASN
27   LYSTHRTYRALAASPVALASNILEASPARG
28   SERARGGLYASPTRPHISVALILELEUTYR
29   LEUMETLYSHISGLYVALTHRASPPROASP
30   LYSILELEUGLULEULEUPROARGASPSER
31   LYSALALYSGLUASNGLULYSTRPASNTHR
32   GLNLYSTYRPHEVALILETHRLEUSERLYS
33   ALATRPSERVALVALLYSLYSTYRLEUGLU
34   ALA

Entity 2, DNA 9 residues - Formula weight is not available

1   DCDTDGDTDGDCDTDCDA

Entity 3, ATP - C10 H16 N5 O13 P3 - 507.181 Da.

1   ATP

Entity 4, dGTP - C10 H16 N5 O13 P3 - 507.181 Da.

1   DGT

Entity 5, MG - Mg - 24.305 Da.

1   MG

Entity 6, ZN - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: protein, [U-99% 15N], 0.2 mM; DNA 0.22 mM; 3'-dATP 0.4 mM; dGTP 0.4 mM; MG 10 mM

sample_conditions_1: ionic strength: 75 mM; pH: 7.0; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D BEST-TROSYsample_1isotropicsample_conditions_1

Software:

AMBER v20 - refinement

TOPSPIN v4.0.7 - collection

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks