BMRB Entry 53264

Name: apo_CTD_BsParB_Relaxation
Published: 2025-07-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53264

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

apo_CTD_BsParB_Relaxation

Deposition date:

2025-07-09

Original release date:

2025-07-14

Authors:

Aleshintsev, Aleksey

Citation:

Citation: Aleshintsev, Aleksey; Way, Lindsey; Guerra, Bianca; Akosua, Lois; Molina, Miranda; Wang, Xindan; Kim, HyeongJun. "ParB C-terminal lysine residues are essential for dimerization, in vitro DNA sliding, and in vivo function." Nucleic Acids Res. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTG011

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QNVPRETKKKEPVKDAVLKE RESYLQNYFGTTVNIKRQKK KGKIEIEFFSNEDLDRILEL LSERES

Data sets:

heteronucl_NOEs
- heteronucl_NOEs_1
heteronucl_T1_relaxation
- heteronucl_T1_relaxation_1
heteronucl_T2_relaxation
- heteronucl_T2_relaxation_1

Data type	Count
T1 relaxation values	59
T2 relaxation values	59
heteronuclear NOE values	58

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CTD_BsParB	1

Entities:

Entity 1, CTD_BsParB 66 residues - Formula weight is not available

1

GLN

ASN

VAL

PRO

ARG

GLU

THR

LYS

2

GLU

PRO

VAL

LYS

ASP

ALA

VAL

LEU

LYS

GLU

3

ARG

GLU

SER

TYR

LEU

GLN

ASN

TYR

PHE

GLY

4

THR

VAL

ASN

ILE

LYS

ARG

GLN

LYS

5

LYS

GLY

LYS

ILE

GLU

ILE

GLU

PHE

SER

6

ASN

GLU

ASP

LEU

ASP

ARG

ILE

LEU

GLU

LEU

7

LEU

SER

GLU

ARG

GLU

SER

Samples:

sample_1: apo_CTD_BsParB, [U-100% 15N], 0.4 mM; D2O 10%; DSS 200 mM; PBS 10 M

sample_conditions_1: pH: 6.1; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
T1/R1 relaxation	sample_1	isotropic	sample_conditions_1
T2/R2 relaxation	sample_1	isotropic	sample_conditions_1
15N-(1H) NOE	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - data analysis

NMR spectrometers:

Bruker AVANCE III 500 MHz