BMRB Entry 53253

Name: 1H 15N backbone chemical shifts 2pY Gab1 613-694
Published: 2025-12-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53253

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H 15N backbone chemical shifts 2pY Gab1 613-694

Deposition date:

2025-07-05

Original release date:

2025-12-16

Authors:

Machner, Lisa; Balbach, Jochen

Citation:

Citation: Machner, Lisa; Shaikhqasem, Alaa; Hamdi, Farzad; Gruber, Tobias; Wiebe, Felix; Breithaupt, Constanze; Kniest, Judith; Lewitzky, Marc; Parthier, Christoph; Kyrilis, Fotios; Balbach, Jochen; Kastritis, Panagiotis; Feller, Stephan; Stubbs, Milton. "Mechanism of SHP2 activation by bis-Tyr-phosphorylated Gab1" Structure ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET42

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SSPMIKPKGDKQVEYLDLDL DSGKSTPPRKQKSSGSGSSV ADERVDYVVVDQQKTLALKS TREAWTDGRQSTESETPAKS VK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	66
1H chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Gab1	1

Entities:

Entity 1, Gab1 82 residues - Formula weight is not available

both tyrosines are phosphorylated

1

SER

PRO

MET

ILE

LYS

PRO

LYS

GLY

ASP

2

LYS

GLN

VAL

GLU

TYR

LEU

ASP

LEU

ASP

LEU

3

ASP

SER

GLY

LYS

SER

THR

PRO

ARG

LYS

4

GLN

LYS

SER

GLY

SER

GLY

SER

VAL

5

ALA

ASP

GLU

ARG

VAL

ASP

TYR

VAL

6

ASP

GLN

LYS

THR

LEU

ALA

LEU

LYS

SER

7

THR

ARG

GLU

ALA

TRP

THR

ASP

GLY

ARG

GLN

8

SER

THR

GLU

SER

GLU

THR

PRO

ALA

LYS

SER

9

VAL

LYS

Samples:

sample_1: free 2pY Gab1 613-694, [U-100% 13C; U-100% 15N], 750 uM; Bis Tris / HCl 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 273.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1

Software:

NMRView - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks