BMRB Entry 53249

Name: Backbone chemical shift assignments for Rattus norvegicus IC-2C (residues 1-96)
Published: 2025-11-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53249

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignments for Rattus norvegicus IC-2C (residues 1-96)

Deposition date:

2025-06-27

Original release date:

2025-11-11

Authors:

Loening, Nikolaus

Citation:

Citation: Di Nicola, AJ; Romig, Bryn; Davis, Stella; Cleary, Paul; Yung, Claire; Marsan, Daniel; Merkt, Anna; Loening, Nikolaus. "Exploration of the interaction between dynein intermediate chain and dynactin p150Glued reveals a novel binding Interface" Protein Sci. 34, e70242-e70242 (2025).
PubMed: 40713938

Assembly members:

Assembly members:
entity_1, polymer, 96 residues, 10868.05 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDKSELKAELERKKQRLAQ IREEKKRKEEERKKKETDQK KEAAVSVQEESDLEKKRREA EALLQSMGLTTDSPIVPPPM SPSSKSVSTPSEAGSQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	88
1H chemical shifts	88

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cytoplasmic dynein 1 intermediate chain 2	1

Entities:

Entity 1, Cytoplasmic dynein 1 intermediate chain 2 96 residues - 10868.05 Da.

1

MET

SER

ASP

LYS

SER

GLU

LEU

LYS

ALA

GLU

2

LEU

GLU

ARG

LYS

GLN

ARG

LEU

ALA

GLN

3

ILE

ARG

GLU

LYS

ARG

LYS

GLU

4

GLU

ARG

LYS

GLU

THR

ASP

GLN

LYS

5

LYS

GLU

ALA

VAL

SER

VAL

GLN

GLU

6

SER

ASP

LEU

GLU

LYS

ARG

GLU

ALA

7

GLU

ALA

LEU

GLN

SER

MET

GLY

LEU

THR

8

THR

ASP

SER

PRO

ILE

VAL

PRO

MET

9

SER

PRO

SER

LYS

SER

VAL

SER

THR

PRO

10

SER

GLU

ALA

GLY

SER

GLN

Samples:

sample_1: Rattus norvegicus dyenin intermediate chain (IC) isoform 2C residues 1-96, [U-99% 15N], 600 ± 30 uM; DSS 200 ± 20 uM; sodium chloride 150 ± 15 mM; sodium phosphate 50 ± 5 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7.4; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS v2.5.2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz