BMRB Entry 53245

Name: Chemical shift assignment of the postsynaptic scaffold protein GKAP C terminal region
Published: 2025-11-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53245

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignment of the postsynaptic scaffold protein GKAP C terminal region

Deposition date:

2025-06-25

Original release date:

2025-11-11

Authors:

Nagy-Kanta, Eszter; Gaspari, Zoltan; Peterfia, Balint; Permi, Perttu

Citation:

Citation: Nagy-Kanta, Eszter; Santa, Anna; Kalman, Zsofia; Li, Jessica Amy; Permi, Perttu; Gaspari, Zoltan; Peterfia, Balint. "1H, 13C, and 15N resonance assignment of the C terminal region of the disordered postsynaptic scaffold protein GKAP" Biomol. NMR Assignments ., .-..
PubMed: 41191219

Assembly members:

Assembly members:
entity_1, polymer, 47 residues, 5246 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMSSQRQEARKRLMAAKR AASVRQNSATESAESIEIYI PEAQTRL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	131
15N chemical shifts	43
1H chemical shifts	177

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rGKAP_950-992	1

Entities:

Entity 1, rGKAP_950-992 47 residues - 5246 Da.

The construct includes 4 residues on the N terminus (GSHM), which are remaining from the non-native affinity tag and the protease cleavage site. However, their chemical shifts are not included in the submitted data.

1

GLY

SER

HIS

MET

SER

GLN

ARG

GLN

GLU

2

ALA

ARG

LYS

ARG

LEU

MET

ALA

LYS

ARG

3

ALA

SER

VAL

ARG

GLN

ASN

SER

ALA

THR

4

GLU

SER

ALA

GLU

SER

ILE

GLU

ILE

TYR

ILE

5

PRO

GLU

ALA

GLN

THR

ARG

LEU

Samples:

sample_1: rGKAP_950-992, [U-100% 15N], 0.3 mM; sodium chloride 20 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D i(HCA)CO(CA)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
4D HACANCOi	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5.7 - collection, processing

CcpNMR v3.1.1 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AVANCE III HD 800 MHz