BMRB Entry 5324

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5324

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Title:
Letters to the Editor: 1H, 15N and 13C assignments of the regulatory domains of a calcium-dependent protein kinase (CDPK)
Deposition date:
2002-03-17
Original release date:
2002-08-23
Authors:
Christodoulou, John; Hu, Haitao; Chung, John; Rance, Mark; Harper, Jeffrey; Chazin, Walter
Citation:

Citation: Christodoulou, John; Hu, Haitao; Chung, John; Rance, Mark; Harper, Jeffrey; Chazin, Walter. "Letters to the Editor: 1H, 15N and 13C assignments of the regulatory domains of a calcium-dependent protein kinase (CDPK)"  J. Biomol. NMR 23, 249-250 (2002).

Assembly members:

Assembly members:
CDPK(428-594), polymer, 170 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CDPK(428-594): GSHMKQFSAMNKFKKMALRV IAESLSEEEIAGLKEMFNMI DADKSGQITFEELKAGLKRV GANLKESEILDLMQAADVDN SGTIDYKEFIAATLHLNKIE REDHLFAAFTYFDKDGSGYI TPDELQQACEEFGVEDVRIE ELMRDVDQDNDGRIDYNEFV AMMQKGSITG

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts452
15N chemical shifts160
1H chemical shifts999

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CDPK(428-594)1

Entities:

Entity 1, CDPK(428-594) 170 residues - Formula weight is not available

1   GLYSERHISMETLYSGLNPHESERALAMET
2   ASNLYSPHELYSLYSMETALALEUARGVAL
3   ILEALAGLUSERLEUSERGLUGLUGLUILE
4   ALAGLYLEULYSGLUMETPHEASNMETILE
5   ASPALAASPLYSSERGLYGLNILETHRPHE
6   GLUGLULEULYSALAGLYLEULYSARGVAL
7   GLYALAASNLEULYSGLUSERGLUILELEU
8   ASPLEUMETGLNALAALAASPVALASPASN
9   SERGLYTHRILEASPTYRLYSGLUPHEILE
10   ALAALATHRLEUHISLEUASNLYSILEGLU
11   ARGGLUASPHISLEUPHEALAALAPHETHR
12   TYRPHEASPLYSASPGLYSERGLYTYRILE
13   THRPROASPGLULEUGLNGLNALACYSGLU
14   GLUPHEGLYVALGLUASPVALARGILEGLU
15   GLULEUMETARGASPVALASPGLNASPASN
16   ASPGLYARGILEASPTYRASNGLUPHEVAL
17   ALAMETMETGLNLYSGLYSERILETHRGLY

Samples:

sample_1: CDPK(428-594)0.5 – 1.0 mM

sample_2: CDPK(428-594), [U-15N], 0.5 – 1.0 mM

sample_3: CDPK(428-594), [U-13C; U-15N], 0.5 – 1.0 mM

sample_4: CDPK(428-594), [U-13C; U-15N; U-2H], 0.5 – 1.0 mM

sample_5: CDPK(428-594), [U-15N]-Lys, 0.5 – 1.0 mM

sample_6: CDPK(428-594), [U-13C]; [U-15N]-Lys, 0.5 – 1.0 mM

Ex-cond_1: pH: 7.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AVANCE 800 MHz
  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

DBJ BAB08991 BAC43300
EMBL CDX70192 CDX80925 CDX98791 CDY11313
GB AAA32761 AAO64867 ABE73345 ABE73346 AED90798
REF NP_196107 XP_002873173 XP_006287286 XP_006398940 XP_009122558
SP Q06850
AlphaFold Q06850

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