BMRB Entry 53232
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53232
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Gonzalez, Lisandro; Hita, Francisco; Pontoriero, Letizia; Pierattelli, Roberta; Binolfi, Andres; Vila, Alejandro. "Periplasmic Protein Quality Control at Atomic Level in Live Cells" Nat. Commun. ., .-..
Assembly members:
entity_1, polymer, 245 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET26
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 517 |
| 15N chemical shifts | 138 |
| 1H chemical shifts | 138 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apoNDM-1 peptides | 1 |
Entities:
Entity 1, apoNDM-1 peptides 245 residues - Formula weight is not available
| 1 | CYS | MET | PRO | GLY | GLU | ILE | ARG | PRO | THR | ILE | ||||
| 2 | GLY | GLN | GLN | MET | GLU | THR | GLY | ASP | GLN | ARG | ||||
| 3 | PHE | GLY | ASP | LEU | VAL | PHE | ARG | GLN | LEU | ALA | ||||
| 4 | PRO | ASN | VAL | TRP | GLN | HIS | THR | SER | TYR | LEU | ||||
| 5 | ASP | MET | PRO | GLY | PHE | GLY | ALA | VAL | ALA | SER | ||||
| 6 | ASN | GLY | LEU | ILE | VAL | ARG | ASP | GLY | GLY | ARG | ||||
| 7 | VAL | LEU | VAL | VAL | ASP | THR | ALA | TRP | THR | ASP | ||||
| 8 | ASP | GLN | THR | ALA | GLN | ILE | LEU | ASN | TRP | ILE | ||||
| 9 | LYS | GLN | GLU | ILE | ASN | LEU | PRO | VAL | ALA | LEU | ||||
| 10 | ALA | VAL | VAL | THR | HIS | ALA | HIS | GLN | ASP | LYS | ||||
| 11 | MET | GLY | GLY | MET | ASP | ALA | LEU | HIS | ALA | ALA | ||||
| 12 | GLY | ILE | ALA | THR | TYR | ALA | ASN | ALA | LEU | SER | ||||
| 13 | ASN | GLN | LEU | ALA | PRO | GLN | GLU | GLY | MET | VAL | ||||
| 14 | ALA | ALA | GLN | HIS | SER | LEU | THR | PHE | ALA | ALA | ||||
| 15 | ASN | GLY | TRP | VAL | GLU | PRO | ALA | THR | ALA | PRO | ||||
| 16 | ASN | PHE | GLY | PRO | LEU | LYS | VAL | PHE | TYR | PRO | ||||
| 17 | GLY | PRO | GLY | HIS | THR | SER | ASP | ASN | ILE | THR | ||||
| 18 | VAL | GLY | ILE | ASP | GLY | THR | ASP | ILE | ALA | PHE | ||||
| 19 | GLY | GLY | CYS | LEU | ILE | LYS | ASP | SER | LYS | ALA | ||||
| 20 | LYS | SER | LEU | GLY | ASN | LEU | GLY | ASP | ALA | ASP | ||||
| 21 | THR | GLU | HIS | TYR | ALA | ALA | SER | ALA | ARG | ALA | ||||
| 22 | PHE | GLY | ALA | ALA | PHE | PRO | LYS | ALA | SER | MET | ||||
| 23 | ILE | VAL | MET | SER | HIS | SER | ALA | PRO | ASP | SER | ||||
| 24 | ARG | ALA | ALA | ILE | THR | HIS | THR | ALA | ARG | MET | ||||
| 25 | ALA | ASP | LYS | LEU | ARG |
Samples:
sample_1: Peptides generated by periplasmic degradation of apo NDM-1, [U-99% 13C; U-99% 15N], 190 uM; D2O, [U-99% 2H], 10%; sodium phosphate 20 mM; sodium chloride 150 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 7; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.9.1.7 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks