BMRB Entry 5323

Name: Solution Structure of the Tenebrio molitor Antifreeze Protein
Published: 2002-05-10

Click here to enlarge.

PDB ID: 1l1i
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5323
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution Structure of the Tenebrio molitor Antifreeze Protein

Deposition date:

2002-03-16

Original release date:

2002-05-10

Authors:

Daley, M.; Spyracopoulos, L.; Jia, Z.; Davies, P.; Sykes, B.

Citation:

Citation: Daley, M.; Spyracopoulos, L.; Jia, Z.; Davies, P.; Sykes, B.. "Structure and Dynamics of a beta-Helical Antifreeze Protein" Biochemistry 41, 5515-5525 (2002).
PubMed: 11969412

Assembly members:

Assembly members:
Tenebrio molitor antifreeze protein, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source: Common Name: yellow mealworm beetle Taxonomy ID: 7067 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Tenebrio molitor

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tenebrio molitor antifreeze protein: QCTGGADCTSCTGACTGCGN CPNAVTCTNSQHCVKANTCT GSTDCNTAQTCTNSKDCFEA NTCTDSTNCYKATACTNSSG CPGH

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
15N chemical shifts	85
1H chemical shifts	420

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Thermal hysteresis protein isoform YL-1 (2-14)	1

Entities:

Entity 1, Thermal hysteresis protein isoform YL-1 (2-14) 84 residues - Formula weight is not available

1

GLN

CYS

THR

GLY

ALA

ASP

CYS

THR

SER

2

CYS

THR

GLY

ALA

CYS

THR

GLY

CYS

GLY

ASN

3

CYS

PRO

ASN

ALA

VAL

THR

CYS

THR

ASN

SER

4

GLN

HIS

CYS

VAL

LYS

ALA

ASN

THR

CYS

THR

5

GLY

SER

THR

ASP

CYS

ASN

THR

ALA

GLN

THR

6

CYS

THR

ASN

SER

LYS

ASP

CYS

PHE

GLU

ALA

7

ASN

THR

CYS

THR

ASP

SER

THR

ASN

CYS

TYR

8

LYS

ALA

THR

ALA

CYS

THR

ASN

SER

GLY

9

CYS

PRO

GLY

HIS

Samples:

sample_1: Tenebrio molitor antifreeze protein, [U-15N], 0.4 mM; H2O 90%; D2O 10%

sample_2: Tenebrio molitor antifreeze protein 1.0 mM; H2O 90%; D2O 10%

sample_3: Tenebrio molitor antifreeze protein 1.0 mM; D2O 100%

sample_cond_1: ionic strength: 0 mM; pH: 5.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-edited TOCSY	not available	not available	not available
HNHA	not available	not available	not available
HNHB	not available	not available	not available
3D 15N-edited NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available
2D NOESY	not available	not available	not available
13C-HSQC	not available	not available	not available
15N-HSQC	not available	not available	not available

Software:

VNMR v6.1B - collection

NMRPipe vsgi6 - processing

NMRView v4.1.2 - data analysis

CNS v1.0 - structure solution

NMR spectrometers:

Varian Unity 600 MHz
Varian INOVA 800 MHz

PDB	1EZG 1L1I
GenBank	AAB70750 ABA46869 ABB29471