BMRB Entry 5321

Name: NMR structure of a SRP19 binding domain in human SRP RNA
Published: 2002-05-20

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PDB ID: 1l1w
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5321
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of a SRP19 binding domain in human SRP RNA

Deposition date:

2002-03-14

Original release date:

2002-05-20

Authors:

Sakamoto, T.; Morita, S.; Tabata, K.; Nakamura, K.; Kawai, G.

Citation:

Citation: Sakamoto, T.; Morita, S.; Tabata, K.; Nakamura, K.; Kawai, G.. "Solution Structure of a SRP19 Binding Domain in Human SRP RNA" J. Biochem. (Tokyo) 132, 177-182 (2002).
PubMed: 12153712

Assembly members:

Assembly members:
helix 6 of signal recognition particle RNA, polymer, 29 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
helix 6 of signal recognition particle RNA: GGUGACCUCCCGGGAGCGGG GGACCACCA

Data sets:

assigned_chemical_shifts
- chemical_shifts_1

Data type	Count
1H chemical shifts	164

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	helix 6 of SRP RNA	1

Entities:

Entity 1, helix 6 of SRP RNA 29 residues - Formula weight is not available

1

G

U

G

A

C

U

C

2

C

G

A

G

C

G

3

G

A

C

A

C

A

Samples:

sample_1: helix 6 of signal recognition particle RNA 0.5 mM; sodium phosphate 20 mM; NaCl 50 mM

sample_2: helix 6 of signal recognition particle RNA, [U-15N; U-13C]-Guanosine,Uridine, 0.5 mM; sodium phosphate 20 mM; NaCl 50 mM

sample_3: helix 6 of signal recognition particle RNA, [U-15N; U-13C]-Adenosine,Cytidine, 0.5 mM; sodium phosphate 20 mM; NaCl 50 mM

sample_4: helix 6 of signal recognition particle RNA, [U-15N; U-13C]-Guanosine,Cytidine, 0.5 mM; sodium phosphate 20 mM; NaCl 50 mM

sample_cond_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
2D HNN-COSY	not available	not available	not available
2D half filtered NOESY	not available	not available	not available
DQF-COSY	not available	not available	not available
2D HP-COSY	not available	not available	not available
2D TOCSY	not available	not available	not available
2D HCCH-COSY	not available	not available	not available
2D HCCH-TOCSY	not available	not available	not available

Software:

xwinnmr v2.1 - collection

VNMR v6.1B - collection

FELIX v97.0 - data analysis

DISCOVER v97.0 - refinement, structure solution

NMR spectrometers:

Bruker DRX 600 MHz
Varian UnityPlus 800 MHz