BMRB Entry 53193

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53193

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Title:
Backbone resonance assignment of asymmetric tetrameric Platelet Factor 4 with low proton split peaks
Deposition date:
2025-05-19
Original release date:
2025-11-08
Authors:
Ma, Qiulin; Huang, Jinfeng; Boulton, Stephen; Mak, Ellen; Akimoto, Madoka; Lopez Giraldo, Estefania; Truong, Mikayla; Daka, Mercy; Huynh, Angela; Clare, Rumi; Kwok, Sarah; Nazy, Ishac; Lee, Woonghee; Melacini, Giuseppe
Citation:

Citation: Ma, Qiulin; Huang, Jinfeng; Boulton, Stephen; Mak, Ellen; Akimoto, Madoka; Lopez Giraldo, Estefania; Truong, Mikayla; Daka, Mercy; Huynh, Angela; Clare, Rumi; Kwok, Sarah; Nazy, Ishac; Lee, Woonghee; Melacini, Giuseppe. "Resonance assignments of asymmetric tetrameric platelet factor 4 (PF4)"  Biomol. NMR Assign. 19, 281-286 (2025).
PubMed: 40921952

Assembly members:

Assembly members:
entity_1, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EAEEDGDLQCLCVKTTSQVR PRHITSLEVIKAGPHCPTAQ LIATLKNGRKICLDLQAPLY KKIIKKLLES

Data sets:
Data typeCount
13C chemical shifts178
15N chemical shifts63
1H chemical shifts63

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PF4 tetramer, chain A1
2PF4 tetramer, chain B1
3PF4 tetramer, chain C1
4PF4 tetramer, chain D1

Entities:

Entity 1, PF4 tetramer, chain A 70 residues - Formula weight is not available

1   GLUALAGLUGLUASPGLYASPLEUGLNCYS
2   LEUCYSVALLYSTHRTHRSERGLNVALARG
3   PROARGHISILETHRSERLEUGLUVALILE
4   LYSALAGLYPROHISCYSPROTHRALAGLN
5   LEUILEALATHRLEULYSASNGLYARGLYS
6   ILECYSLEUASPLEUGLNALAPROLEUTYR
7   LYSLYSILEILELYSLYSLEULEUGLUSER

Samples:

sample_1: PF4 tetramer, [U-100% 15N], 160 uM; PF4 tetramer, [U-100% 13C; U-100% 15N], 160 uM; HEPES buffer 20 mM; NaCl 400 mM

sample_conditions_1: ionic strength: 400 mM; pH: 7.2; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking

NMRPipe - processing

TOPSPIN - processing

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks