BMRB Entry 53188

Name: EB1 EBH(191-260) bound to MACF peptide, relaxation data
Published: 2025-12-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53188

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

EB1 EBH(191-260) bound to MACF peptide, relaxation data

Deposition date:

2025-05-16

Original release date:

2025-12-09

Authors:

Barsukov, Igor; Hargreaves, Eleanor

Citation:

Citation: Almeida, Teresa; Hargreaves, Eleanor; Zech, Tobias; Barsukov, Igor. "Dock-and-lock binding of SxIP ligands is required for stable and selective EB1 interactions" eLife ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 70 residues, 8049.979 Da.
entity_2, polymer, 11 residues, 1283.561 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pOPINS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DEAAELMQQVNVLKLTVEDL EKERDFYFGKLRNIELICQE NEGENDPVLQRIVDILYATD EGFVIPDEGG
entity_2: KPSKIPTPQRK

Data sets:

heteronucl_NOEs
- heteronucl_NOEs_1
- heteronucl_NOEs_2
heteronucl_T1_relaxation
- heteronucl_T1_relaxation_1
- heteronucl_T1_relaxation_2
heteronucl_T2_relaxation
- heteronucl_T2_relaxation_1
- heteronucl_T2_relaxation_2

Data type	Count
T1 relaxation values	132
T2 relaxation values	132
heteronuclear NOE values	132

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1, 1	1
2	entity_1, 2	1
3	entity_2, 1	2
4	entity_2, 2	2

Entities:

Entity 1, entity_1, 1 70 residues - 8049.979 Da.

1	ASP	GLU	ALA	ALA	GLU	LEU	MET	GLN	GLN	VAL
2	ASN	VAL	LEU	LYS	LEU	THR	VAL	GLU	ASP	LEU
3	GLU	LYS	GLU	ARG	ASP	PHE	TYR	PHE	GLY	LYS
4	LEU	ARG	ASN	ILE	GLU	LEU	ILE	CYS	GLN	GLU
5	ASN	GLU	GLY	GLU	ASN	ASP	PRO	VAL	LEU	GLN
6	ARG	ILE	VAL	ASP	ILE	LEU	TYR	ALA	THR	ASP
7	GLU	GLY	PHE	VAL	ILE	PRO	ASP	GLU	GLY	GLY

Entity 2, entity_2, 1 11 residues - 1283.561 Da.

1

LYS

PRO

SER

LYS

ILE

PRO

THR

PRO

GLN

ARG

2

LYS

Samples:

sample_1: entity_1, [U-15N], 0.63 mM; entity_2 3 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N T1-HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N T2-HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N heteronoe	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N T1-HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N T2-HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N heteronoe	sample_1	isotropic	sample_conditions_1

Software:

Relax v5.0.0 - relaxation analysis

ARIA - structure calculation

CcpNMR - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AvanceIII 600 MHz
Bruker AvanceIII 800 MHz

1	ASP	GLU	ALA	ALA	GLU	LEU	MET	GLN	GLN	VAL
2	ASN	VAL	LEU	LYS	LEU	THR	VAL	GLU	ASP	LEU
3	GLU	LYS	GLU	ARG	ASP	PHE	TYR	PHE	GLY	LYS
4	LEU	ARG	ASN	ILE	GLU	LEU	ILE	CYS	GLN	GLU
5	ASN	GLU	GLY	GLU	ASN	ASP	PRO	VAL	LEU	GLN
6	ARG	ILE	VAL	ASP	ILE	LEU	TYR	ALA	THR	ASP
7	GLU	GLY	PHE	VAL	ILE	PRO	ASP	GLU	GLY	GLY

1	ASP	GLU	ALA	ALA	GLU	LEU	MET	GLN	GLN	VAL
2	ASN	VAL	LEU	LYS	LEU	THR	VAL	GLU	ASP	LEU
3	GLU	LYS	GLU	ARG	ASP	PHE	TYR	PHE	GLY	LYS
4	LEU	ARG	ASN	ILE	GLU	LEU	ILE	CYS	GLN	GLU
5	ASN	GLU	GLY	GLU	ASN	ASP	PRO	VAL	LEU	GLN
6	ARG	ILE	VAL	ASP	ILE	LEU	TYR	ALA	THR	ASP
7	GLU	GLY	PHE	VAL	ILE	PRO	ASP	GLU	GLY	GLY