BMRB Entry 5316

Name: Structure of the N-terminal 283-Residue Fragment of the HIV-1 Gag Polyprotein
Published: 2003-10-06

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PDB ID: 1l6n
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5316
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of the N-terminal 283-Residue Fragment of the HIV-1 Gag Polyprotein

Deposition date:

2002-03-09

Original release date:

2003-10-06

Authors:

Tang, Chun; Ndassa, Yasmine; Summers, Michael

Citation:

Citation: Tang, Chun; Ndassa, Yasmine; Summers, Michael. "Structure of the N-terminal 283-Residue Fragment of the HIV-1 Gag Polyprotein" Nat. Struct. Biol. 9, 537-543 (2002).
PubMed: 12032547

Assembly members:

Assembly members:
matrix-capsid, polymer, 288 residues, 32216.6 Da.

Natural source:

Natural source: Common Name: HIV Taxonomy ID: 12721 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus Human immunodeficiency virus type 1

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
matrix-capsid: GARASVLSGGELDKWEKIRL RPGGKKQYKLKHIVWASREL ERFAVNPGLLETSEGCRQIL GQLQPSLQTGSEELRSLYNT IAVLYCVHQRIDVKDTKEAL DKIEEEQNKSKKKAQQAAAD TGNNSQVSQNYPIVQNLQGQ MVHQAISPRTLNAWVKVVEE KAFSPEVIPMFSALSEGATP QDLNTMLNTVGGHQAAMQML KETINEEAAEWDRLHPVHAG PIAPGQMREPRGSDIAGTTS TLQEQIGWMTHNPPIPVGEI YKRWIILGLNKIVRMYSPTS ILHHHHHH

Data sets:

assigned_chemical_shifts
- shift_set_1
- shift_set_2

Data type	Count
13C chemical shifts	1138
15N chemical shifts	273
1H chemical shifts	1636

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Gag283	1

Entities:

Entity 1, Gag283 288 residues - 32216.6 Da.

1

GLY

ALA

ARG

ALA

SER

VAL

LEU

SER

GLY

2

GLU

LEU

ASP

LYS

TRP

GLU

LYS

ILE

ARG

LEU

3

ARG

PRO

GLY

LYS

GLN

TYR

LYS

LEU

4

LYS

HIS

ILE

VAL

TRP

ALA

SER

ARG

GLU

LEU

5

GLU

ARG

PHE

ALA

VAL

ASN

PRO

GLY

LEU

6

GLU

THR

SER

GLU

GLY

CYS

ARG

GLN

ILE

LEU

7

GLY

GLN

LEU

GLN

PRO

SER

LEU

GLN

THR

GLY

8

SER

GLU

LEU

ARG

SER

LEU

TYR

ASN

THR

9

ILE

ALA

VAL

LEU

TYR

CYS

VAL

HIS

GLN

ARG

10

ILE

ASP

VAL

LYS

ASP

THR

LYS

GLU

ALA

LEU

11

ASP

LYS

ILE

GLU

GLN

ASN

LYS

SER

12

LYS

ALA

GLN

ALA

ASP

13

THR

GLY

ASN

SER

GLN

VAL

SER

GLN

ASN

14

TYR

PRO

ILE

VAL

GLN

ASN

LEU

GLN

GLY

GLN

15

MET

VAL

HIS

GLN

ALA

ILE

SER

PRO

ARG

THR

16

LEU

ASN

ALA

TRP

VAL

LYS

VAL

GLU

17

LYS

ALA

PHE

SER

PRO

GLU

VAL

ILE

PRO

MET

18

PHE

SER

ALA

LEU

SER

GLU

GLY

ALA

THR

PRO

19

GLN

ASP

LEU

ASN

THR

MET

LEU

ASN

THR

VAL

20

GLY

HIS

GLN

ALA

MET

GLN

MET

LEU

21

LYS

GLU

THR

ILE

ASN

GLU

ALA

GLU

22

TRP

ASP

ARG

LEU

HIS

PRO

VAL

HIS

ALA

GLY

23

PRO

ILE

ALA

PRO

GLY

GLN

MET

ARG

GLU

PRO

24

ARG

GLY

SER

ASP

ILE

ALA

GLY

THR

SER

25

THR

LEU

GLN

GLU

GLN

ILE

GLY

TRP

MET

THR

26

HIS

ASN

PRO

ILE

PRO

VAL

GLY

GLU

ILE

27

TYR

LYS

ARG

TRP

ILE

LEU

GLY

LEU

ASN

28

LYS

ILE

VAL

ARG

MET

TYR

SER

PRO

THR

SER

29

ILE

LEU

HIS

Samples:

sample_1: matrix-capsid, [U-100% 13C; U-100% 15N], 1.0 mM; sodium acetate 50 mM; NaCl 100 mM; beta-mecaptoethanol 5 mM

cond_1: ionic strength: 0.15 M; pH: 5.0; temperature: 303.3 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
1H-15N TROSY	not available	not available	not available
15N-edited NOESY-HSQC	not available	not available	not available
15N-edited TOCSY-HSQC	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCO	not available	not available	not available
HNHA	not available	not available	not available
15N,15N-edited HSQC-NOESY-HSQC	not available	not available	not available
15N,13C-edited HSQC-NOESY-HMQC	not available	not available	not available
13C,13C-edited HMQC-NOESY-HMQC	not available	not available	not available

Software:

NMRPIPE - spectra processing

NMRDRAW - spectra processing

NMRVIEW v5.0.3 - spectra analysis

NMR spectrometers:

Bruker DMX 600 MHz

PDB	1L6N
GenBank	AAA44987 AAB60571 AAC28445 AAK08483 AAX32941
SWISS-PROT	P12493 P12497