BMRB Entry 53136
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53136
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Citation: Lai, Chih-Hsuan; Lou, Yuan-Chao; Chang, Chi-Fon; Lu, Wei-Lin; Sriramoju, Manoj Kumar; Wang, Yong-Sheng; Wu, Kuen-Phon; Camilloni, Carlo; Hsu, Shang-Te Danny. "Allosteric network of dynamic coupling within BAP1-UCH revealed by methyl NMR
" Nat. Commun. 17, 6867-6867 (2026).
PubMed: 42191708
Assembly members:
BAP1-UCH_F15L, polymer, 241 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 50 |
| 1H chemical shifts | 150 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BAP1-UCH | 1 |
Entities:
Entity 1, BAP1-UCH 241 residues - Formula weight is not available
Residues 1-3 are the cleavage sequence for TEV protease. Met1 of BAP1-UCH starts from residue 4.
| 1 | SER | ASN | GLY | MET | ASN | LYS | GLY | TRP | LEU | GLU | ||||
| 2 | LEU | GLU | SER | ASP | PRO | GLY | LEU | LEU | THR | LEU | ||||
| 3 | LEU | VAL | GLU | ASP | PHE | GLY | VAL | LYS | GLY | VAL | ||||
| 4 | GLN | VAL | GLU | GLU | ILE | TYR | ASP | LEU | GLN | SER | ||||
| 5 | LYS | CYS | GLN | GLY | PRO | VAL | TYR | GLY | PHE | ILE | ||||
| 6 | PHE | LEU | PHE | LYS | TRP | ILE | GLU | GLU | ARG | ARG | ||||
| 7 | SER | ARG | ARG | LYS | VAL | SER | THR | LEU | VAL | ASP | ||||
| 8 | ASP | THR | SER | VAL | ILE | ASP | ASP | ASP | ILE | VAL | ||||
| 9 | ASN | ASN | MET | PHE | PHE | ALA | HIS | GLN | LEU | ILE | ||||
| 10 | PRO | ASN | SER | CYS | ALA | THR | HIS | ALA | LEU | LEU | ||||
| 11 | SER | VAL | LEU | LEU | ASN | CYS | SER | SER | VAL | ASP | ||||
| 12 | LEU | GLY | PRO | THR | LEU | SER | ARG | MET | LYS | ASP | ||||
| 13 | PHE | THR | LYS | GLY | PHE | SER | PRO | GLU | SER | LYS | ||||
| 14 | GLY | TYR | ALA | ILE | GLY | ASN | ALA | PRO | GLU | LEU | ||||
| 15 | ALA | LYS | ALA | HIS | ASN | SER | HIS | ALA | ARG | PRO | ||||
| 16 | GLU | PRO | ARG | HIS | LEU | PRO | GLU | LYS | GLN | ASN | ||||
| 17 | GLY | LEU | SER | ALA | VAL | ARG | THR | MET | GLU | ALA | ||||
| 18 | PHE | HIS | PHE | VAL | SER | TYR | VAL | PRO | ILE | THR | ||||
| 19 | GLY | ARG | LEU | PHE | GLU | LEU | ASP | GLY | LEU | LYS | ||||
| 20 | VAL | TYR | PRO | ILE | ASP | HIS | GLY | PRO | TRP | GLY | ||||
| 21 | GLU | ASP | GLU | GLU | TRP | THR | ASP | LYS | ALA | ARG | ||||
| 22 | ARG | VAL | ILE | MET | GLU | ARG | ILE | GLY | LEU | ALA | ||||
| 23 | THR | ALA | GLY | GLU | PRO | TYR | HIS | ASP | ILE | ARG | ||||
| 24 | PHE | ASN | LEU | MET | ALA | VAL | VAL | PRO | ASP | ARG | ||||
| 25 | ARG |
Samples:
sample_1: BAP1-UCH_F15L, [U-13C], 145 uM; MOPS 50 mM; NaCl 50 mM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
POKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz