BMRB Entry 53127

Name: Backbone 1H, 15N, 13C Resonance Assignment of p47
Published: 2025-11-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53127

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 15N, 13C Resonance Assignment of p47

Deposition date:

2025-05-07

Original release date:

2025-11-12

Authors:

Raseekan, Rajivan; Black, Megan; Huang, Rui

Citation:

Citation: Raseekan, Rajivan; Black, Megan; Huang, Rui. "1H, 13C, and 15N backbone resonance assignments of full-length and a SIM-SEP-containing fragment (101-266) of p47, a p97 adaptor protein" Biomol. NMR Assign. 20, 1-1 (2025).
PubMed: 41188554

Assembly members:

Assembly members:
entity_1, polymer, 370 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAEERQDALREFVAVTGTEE DRARFFLESAGWDLQIALAS FYEDGGDEDIVTISQATPSS VSRGTAPSDNRVTSFRDLIH DQDEEEEEEEGQRFYAGGSE RSGQQIVGPPRKKSPNELVD DLFKGAKEHGAVAVERVTKS PGETSKPRPFAGGGYRLGAA PEEESAYVAGERRRHSGQDV HVVLKLWKTGFSLDNGDLRS YQDPSNAQFLESIRRGEVPA ELRRLAHGGQVNLDMEDHRD EDFVKPKGAFKAFTGEGQKL GSTAPQVLNTSSPAQQAENE AKASSSILINEAEPTTNIQI RLADGGRLVQKFNHSHRISD IRLFIVDARPAMAATSFVLM TTFPNKELADENQTLKEANL LNAVIVQRLT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	951
15N chemical shifts	316
1H chemical shifts	316

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p47	1

Entities:

Entity 1, p47 370 residues - Formula weight is not available

1	MET	ALA	GLU	GLU	ARG	GLN	ASP	ALA	LEU	ARG
2	GLU	PHE	VAL	ALA	VAL	THR	GLY	THR	GLU	GLU
3	ASP	ARG	ALA	ARG	PHE	PHE	LEU	GLU	SER	ALA
4	GLY	TRP	ASP	LEU	GLN	ILE	ALA	LEU	ALA	SER
5	PHE	TYR	GLU	ASP	GLY	GLY	ASP	GLU	ASP	ILE
6	VAL	THR	ILE	SER	GLN	ALA	THR	PRO	SER	SER
7	VAL	SER	ARG	GLY	THR	ALA	PRO	SER	ASP	ASN
8	ARG	VAL	THR	SER	PHE	ARG	ASP	LEU	ILE	HIS
9	ASP	GLN	ASP	GLU	GLU	GLU	GLU	GLU	GLU	GLU
10	GLY	GLN	ARG	PHE	TYR	ALA	GLY	GLY	SER	GLU
11	ARG	SER	GLY	GLN	GLN	ILE	VAL	GLY	PRO	PRO
12	ARG	LYS	LYS	SER	PRO	ASN	GLU	LEU	VAL	ASP
13	ASP	LEU	PHE	LYS	GLY	ALA	LYS	GLU	HIS	GLY
14	ALA	VAL	ALA	VAL	GLU	ARG	VAL	THR	LYS	SER
15	PRO	GLY	GLU	THR	SER	LYS	PRO	ARG	PRO	PHE
16	ALA	GLY	GLY	GLY	TYR	ARG	LEU	GLY	ALA	ALA
17	PRO	GLU	GLU	GLU	SER	ALA	TYR	VAL	ALA	GLY
18	GLU	ARG	ARG	ARG	HIS	SER	GLY	GLN	ASP	VAL
19	HIS	VAL	VAL	LEU	LYS	LEU	TRP	LYS	THR	GLY
20	PHE	SER	LEU	ASP	ASN	GLY	ASP	LEU	ARG	SER
21	TYR	GLN	ASP	PRO	SER	ASN	ALA	GLN	PHE	LEU
22	GLU	SER	ILE	ARG	ARG	GLY	GLU	VAL	PRO	ALA
23	GLU	LEU	ARG	ARG	LEU	ALA	HIS	GLY	GLY	GLN
24	VAL	ASN	LEU	ASP	MET	GLU	ASP	HIS	ARG	ASP
25	GLU	ASP	PHE	VAL	LYS	PRO	LYS	GLY	ALA	PHE
26	LYS	ALA	PHE	THR	GLY	GLU	GLY	GLN	LYS	LEU
27	GLY	SER	THR	ALA	PRO	GLN	VAL	LEU	ASN	THR
28	SER	SER	PRO	ALA	GLN	GLN	ALA	GLU	ASN	GLU
29	ALA	LYS	ALA	SER	SER	SER	ILE	LEU	ILE	ASN
30	GLU	ALA	GLU	PRO	THR	THR	ASN	ILE	GLN	ILE
31	ARG	LEU	ALA	ASP	GLY	GLY	ARG	LEU	VAL	GLN
32	LYS	PHE	ASN	HIS	SER	HIS	ARG	ILE	SER	ASP
33	ILE	ARG	LEU	PHE	ILE	VAL	ASP	ALA	ARG	PRO
34	ALA	MET	ALA	ALA	THR	SER	PHE	VAL	LEU	MET
35	THR	THR	PHE	PRO	ASN	LYS	GLU	LEU	ALA	ASP
36	GLU	ASN	GLN	THR	LEU	LYS	GLU	ALA	ASN	LEU
37	LEU	ASN	ALA	VAL	ILE	VAL	GLN	ARG	LEU	THR

Samples:

sample_1: p47, [U-13C; U-15N; U-2H], 639 uM; Bis-Tris 25 mM; EDTA 1 mM; TCEP 3 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.6; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v3.2.12 - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	ALA	GLU	GLU	ARG	GLN	ASP	ALA	LEU	ARG
2	GLU	PHE	VAL	ALA	VAL	THR	GLY	THR	GLU	GLU
3	ASP	ARG	ALA	ARG	PHE	PHE	LEU	GLU	SER	ALA
4	GLY	TRP	ASP	LEU	GLN	ILE	ALA	LEU	ALA	SER
5	PHE	TYR	GLU	ASP	GLY	GLY	ASP	GLU	ASP	ILE
6	VAL	THR	ILE	SER	GLN	ALA	THR	PRO	SER	SER
7	VAL	SER	ARG	GLY	THR	ALA	PRO	SER	ASP	ASN
8	ARG	VAL	THR	SER	PHE	ARG	ASP	LEU	ILE	HIS
9	ASP	GLN	ASP	GLU	GLU	GLU	GLU	GLU	GLU	GLU
10	GLY	GLN	ARG	PHE	TYR	ALA	GLY	GLY	SER	GLU
11	ARG	SER	GLY	GLN	GLN	ILE	VAL	GLY	PRO	PRO
12	ARG	LYS	LYS	SER	PRO	ASN	GLU	LEU	VAL	ASP
13	ASP	LEU	PHE	LYS	GLY	ALA	LYS	GLU	HIS	GLY
14	ALA	VAL	ALA	VAL	GLU	ARG	VAL	THR	LYS	SER
15	PRO	GLY	GLU	THR	SER	LYS	PRO	ARG	PRO	PHE
16	ALA	GLY	GLY	GLY	TYR	ARG	LEU	GLY	ALA	ALA
17	PRO	GLU	GLU	GLU	SER	ALA	TYR	VAL	ALA	GLY
18	GLU	ARG	ARG	ARG	HIS	SER	GLY	GLN	ASP	VAL
19	HIS	VAL	VAL	LEU	LYS	LEU	TRP	LYS	THR	GLY
20	PHE	SER	LEU	ASP	ASN	GLY	ASP	LEU	ARG	SER
21	TYR	GLN	ASP	PRO	SER	ASN	ALA	GLN	PHE	LEU
22	GLU	SER	ILE	ARG	ARG	GLY	GLU	VAL	PRO	ALA
23	GLU	LEU	ARG	ARG	LEU	ALA	HIS	GLY	GLY	GLN
24	VAL	ASN	LEU	ASP	MET	GLU	ASP	HIS	ARG	ASP
25	GLU	ASP	PHE	VAL	LYS	PRO	LYS	GLY	ALA	PHE
26	LYS	ALA	PHE	THR	GLY	GLU	GLY	GLN	LYS	LEU
27	GLY	SER	THR	ALA	PRO	GLN	VAL	LEU	ASN	THR
28	SER	SER	PRO	ALA	GLN	GLN	ALA	GLU	ASN	GLU
29	ALA	LYS	ALA	SER	SER	SER	ILE	LEU	ILE	ASN
30	GLU	ALA	GLU	PRO	THR	THR	ASN	ILE	GLN	ILE
31	ARG	LEU	ALA	ASP	GLY	GLY	ARG	LEU	VAL	GLN
32	LYS	PHE	ASN	HIS	SER	HIS	ARG	ILE	SER	ASP
33	ILE	ARG	LEU	PHE	ILE	VAL	ASP	ALA	ARG	PRO
34	ALA	MET	ALA	ALA	THR	SER	PHE	VAL	LEU	MET
35	THR	THR	PHE	PRO	ASN	LYS	GLU	LEU	ALA	ASP
36	GLU	ASN	GLN	THR	LEU	LYS	GLU	ALA	ASN	LEU
37	LEU	ASN	ALA	VAL	ILE	VAL	GLN	ARG	LEU	THR

1	MET	ALA	GLU	GLU	ARG	GLN	ASP	ALA	LEU	ARG
2	GLU	PHE	VAL	ALA	VAL	THR	GLY	THR	GLU	GLU
3	ASP	ARG	ALA	ARG	PHE	PHE	LEU	GLU	SER	ALA
4	GLY	TRP	ASP	LEU	GLN	ILE	ALA	LEU	ALA	SER
5	PHE	TYR	GLU	ASP	GLY	GLY	ASP	GLU	ASP	ILE
6	VAL	THR	ILE	SER	GLN	ALA	THR	PRO	SER	SER
7	VAL	SER	ARG	GLY	THR	ALA	PRO	SER	ASP	ASN
8	ARG	VAL	THR	SER	PHE	ARG	ASP	LEU	ILE	HIS
9	ASP	GLN	ASP	GLU	GLU	GLU	GLU	GLU	GLU	GLU
10	GLY	GLN	ARG	PHE	TYR	ALA	GLY	GLY	SER	GLU
11	ARG	SER	GLY	GLN	GLN	ILE	VAL	GLY	PRO	PRO
12	ARG	LYS	LYS	SER	PRO	ASN	GLU	LEU	VAL	ASP
13	ASP	LEU	PHE	LYS	GLY	ALA	LYS	GLU	HIS	GLY
14	ALA	VAL	ALA	VAL	GLU	ARG	VAL	THR	LYS	SER
15	PRO	GLY	GLU	THR	SER	LYS	PRO	ARG	PRO	PHE
16	ALA	GLY	GLY	GLY	TYR	ARG	LEU	GLY	ALA	ALA
17	PRO	GLU	GLU	GLU	SER	ALA	TYR	VAL	ALA	GLY
18	GLU	ARG	ARG	ARG	HIS	SER	GLY	GLN	ASP	VAL
19	HIS	VAL	VAL	LEU	LYS	LEU	TRP	LYS	THR	GLY
20	PHE	SER	LEU	ASP	ASN	GLY	ASP	LEU	ARG	SER
21	TYR	GLN	ASP	PRO	SER	ASN	ALA	GLN	PHE	LEU
22	GLU	SER	ILE	ARG	ARG	GLY	GLU	VAL	PRO	ALA
23	GLU	LEU	ARG	ARG	LEU	ALA	HIS	GLY	GLY	GLN
24	VAL	ASN	LEU	ASP	MET	GLU	ASP	HIS	ARG	ASP
25	GLU	ASP	PHE	VAL	LYS	PRO	LYS	GLY	ALA	PHE
26	LYS	ALA	PHE	THR	GLY	GLU	GLY	GLN	LYS	LEU
27	GLY	SER	THR	ALA	PRO	GLN	VAL	LEU	ASN	THR
28	SER	SER	PRO	ALA	GLN	GLN	ALA	GLU	ASN	GLU
29	ALA	LYS	ALA	SER	SER	SER	ILE	LEU	ILE	ASN
30	GLU	ALA	GLU	PRO	THR	THR	ASN	ILE	GLN	ILE
31	ARG	LEU	ALA	ASP	GLY	GLY	ARG	LEU	VAL	GLN
32	LYS	PHE	ASN	HIS	SER	HIS	ARG	ILE	SER	ASP
33	ILE	ARG	LEU	PHE	ILE	VAL	ASP	ALA	ARG	PRO
34	ALA	MET	ALA	ALA	THR	SER	PHE	VAL	LEU	MET
35	THR	THR	PHE	PRO	ASN	LYS	GLU	LEU	ALA	ASP
36	GLU	ASN	GLN	THR	LEU	LYS	GLU	ALA	ASN	LEU
37	LEU	ASN	ALA	VAL	ILE	VAL	GLN	ARG	LEU	THR

1	MET	ALA	GLU	GLU	ARG	GLN	ASP	ALA	LEU	ARG
2	GLU	PHE	VAL	ALA	VAL	THR	GLY	THR	GLU	GLU
3	ASP	ARG	ALA	ARG	PHE	PHE	LEU	GLU	SER	ALA
4	GLY	TRP	ASP	LEU	GLN	ILE	ALA	LEU	ALA	SER
5	PHE	TYR	GLU	ASP	GLY	GLY	ASP	GLU	ASP	ILE
6	VAL	THR	ILE	SER	GLN	ALA	THR	PRO	SER	SER
7	VAL	SER	ARG	GLY	THR	ALA	PRO	SER	ASP	ASN
8	ARG	VAL	THR	SER	PHE	ARG	ASP	LEU	ILE	HIS
9	ASP	GLN	ASP	GLU	GLU	GLU	GLU	GLU	GLU	GLU
10	GLY	GLN	ARG	PHE	TYR	ALA	GLY	GLY	SER	GLU
11	ARG	SER	GLY	GLN	GLN	ILE	VAL	GLY	PRO	PRO
12	ARG	LYS	LYS	SER	PRO	ASN	GLU	LEU	VAL	ASP
13	ASP	LEU	PHE	LYS	GLY	ALA	LYS	GLU	HIS	GLY
14	ALA	VAL	ALA	VAL	GLU	ARG	VAL	THR	LYS	SER
15	PRO	GLY	GLU	THR	SER	LYS	PRO	ARG	PRO	PHE
16	ALA	GLY	GLY	GLY	TYR	ARG	LEU	GLY	ALA	ALA
17	PRO	GLU	GLU	GLU	SER	ALA	TYR	VAL	ALA	GLY
18	GLU	ARG	ARG	ARG	HIS	SER	GLY	GLN	ASP	VAL
19	HIS	VAL	VAL	LEU	LYS	LEU	TRP	LYS	THR	GLY
20	PHE	SER	LEU	ASP	ASN	GLY	ASP	LEU	ARG	SER
21	TYR	GLN	ASP	PRO	SER	ASN	ALA	GLN	PHE	LEU
22	GLU	SER	ILE	ARG	ARG	GLY	GLU	VAL	PRO	ALA
23	GLU	LEU	ARG	ARG	LEU	ALA	HIS	GLY	GLY	GLN
24	VAL	ASN	LEU	ASP	MET	GLU	ASP	HIS	ARG	ASP
25	GLU	ASP	PHE	VAL	LYS	PRO	LYS	GLY	ALA	PHE
26	LYS	ALA	PHE	THR	GLY	GLU	GLY	GLN	LYS	LEU
27	GLY	SER	THR	ALA	PRO	GLN	VAL	LEU	ASN	THR
28	SER	SER	PRO	ALA	GLN	GLN	ALA	GLU	ASN	GLU
29	ALA	LYS	ALA	SER	SER	SER	ILE	LEU	ILE	ASN
30	GLU	ALA	GLU	PRO	THR	THR	ASN	ILE	GLN	ILE
31	ARG	LEU	ALA	ASP	GLY	GLY	ARG	LEU	VAL	GLN
32	LYS	PHE	ASN	HIS	SER	HIS	ARG	ILE	SER	ASP
33	ILE	ARG	LEU	PHE	ILE	VAL	ASP	ALA	ARG	PRO
34	ALA	MET	ALA	ALA	THR	SER	PHE	VAL	LEU	MET
35	THR	THR	PHE	PRO	ASN	LYS	GLU	LEU	ALA	ASP
36	GLU	ASN	GLN	THR	LEU	LYS	GLU	ALA	ASN	LEU
37	LEU	ASN	ALA	VAL	ILE	VAL	GLN	ARG	LEU	THR